CAJ | 학술논문

背景:长期临床研究表明黄芪三仙汤对骨质疏松症有一定的治疗作用,基础实验研究也表明黄芪三仙汤能改善实验动物的生物力学指标.在此基础上实验拟从细胞分子水平上探讨黄芪三仙汤对成骨细胞的相关作用.目的:观察黄芪三仙汤对体外培养成骨细胞护骨素、护骨素配体基因调节的作用,探讨黄芪三仙汤治疗骨质疏松的作用机制.方法:制备黄芪三仙汤和仙灵骨葆胶囊含药血清及空白血清,对体外培养新生 SD 大鼠成骨细胞进行干预,应用细胞形态观察、MTT、Western blot 分析法及蛋白浓度测定法,观察各含药血清对体外培养成骨细胞的增殖及成骨细胞护骨素、护骨素配体表达的影响.结果与结论:经药物干预后成骨细胞变为长梭形、条索状,融合成片,分界较为模糊,细胞生长较密集.黄芪三仙汤可明显促进成骨细胞护骨素蛋白浓度的提高(P <0.01),同时也提高了护骨素配体蛋白浓度(P <0.01),而护骨素/护骨素配体浓度比例水平明显升高(P <0.01).结果可见黄芪三仙汤可能是通过调节成骨细胞分化调控因子护骨素、护骨素配体的表达来调节成骨细胞的增长,从而实现其对骨质疏松症的治疗作用.
배경:장기림상연구표명황기삼선탕대골질소송증유일정적치료작용,기출실험연구야표명황기삼선탕능개선실험동물적생물역학지표.재차기출상실험의종세포분자수평상탐토황기삼선탕대성골세포적상관작용.목적:관찰황기삼선탕대체외배양성골세포호골소、호골소배체기인조절적작용,탐토황기삼선탕치료골질소송적작용궤제.방법:제비황기삼선탕화선령골보효낭함약혈청급공백혈청,대체외배양신생 SD 대서성골세포진행간예,응용세포형태관찰、MTT、Western blot 분석법급단백농도측정법,관찰각함약혈청대체외배양성골세포적증식급성골세포호골소、호골소배체표체적영향.결과여결론:경약물간예후성골세포변위장사형、조색상,융합성편,분계교위모호,세포생장교밀집.황기삼선탕가명현촉진성골세포호골소단백농도적제고(P <0.01),동시야제고료호골소배체단백농도(P <0.01),이호골소/호골소배체농도비례수평명현승고(P <0.01).결과가견황기삼선탕가능시통과조절성골세포분화조공인자호골소、호골소배체적표체래조절성골세포적증장,종이실현기대골질소송증적치료작용.
BACKGROUND: Long-term clinical research has demonstrated that Huangqi Sanxian decoction exerts a certain curative effect on osteoporosis. In addition, experimental studies have also showed that Huangqi Sanxian decoction can improve the biomechanical indexes in experimental animals. The present study aimed to investigate the effect of Huangqi Sanxian decoction on osteoblasts in the cel ular level. OBJECTIVE: To observe the effects of Huangqi Sanxian decoction on gene regulation of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in osteoblasts cultured in vitro and to investigate the action mechanism underlying the curative effect of Huangqi Sanxian decoction on osteoporosis. METHODS: Huangqi Sanxian decoction, Xianlinggubao capsule containing serum and blank serum were prepared to intervening osteoblasts from newborn Sprague-Dawley rats cultured in vitro. Cel ular morphology was observed, and western blot analysis and determination of protein concentration were used to observe the effects of drug-containing serum on osteoblast proliferation, gene expression of osteoprotegerin and receptor activator of nuclear factor kappa B ligand in osteoblasts cultured in vitro. RESULTS AND CONCLUSION: After drug intervention, osteoblasts were spindle-shaped and trabs-shaped, which were concentrated and confluent with undefined boundary. Huangqi Sanxian decoction could increased the protein expression of osteoprotegerin in osteoblasts (P < 0.01), and also increased the protein expression of receptor activator of nuclear factor kappa B ligand (P < 0.01) to increase ratio of osteoprotegerin/receptor activator of nuclear factor kappa B ligand (P < 0.01). Huangqi Sanxian decoction can achieve the therapeutic effect on osteoporosis through regulating the expression of osteoprotegerin and receptor activator of nuclear factor kappa B ligand.