CAJ | 학술논문

背景:小鼠的下颌下腺是研究唾液腺的发育的良好模型,转化生长因子β是器官发育和疾病中重要的生长因子,但是在下颌下腺中转化生长因子β受体的表达以及作用机制至今并不明确.目的:观察胚胎小鼠下颌下腺发育过程中转化生长因子βⅠ型受体和Ⅱ型受体以及 p-ERK1/2的表达,揭示转化生长因子β在小鼠涎腺发育中的作用.方法:取 C57BL/6J 小鼠胚胎期第14.5天的标本,使用转化生长因子βⅠ型受体和Ⅱ型受体以及p-ERK1/2抗体,对小鼠的下颌下腺进行免疫组化染色.取新生小鼠标本,大体观察下颌下腺,并且使用苏木精-伊红染色观察其形态.结果与结论:①小鼠出生时,下颌下腺位于下颌骨下方;苏木精-伊红染色发现小鼠下颌下腺的腺泡、导管和闰管细胞也已经分化完成.②在胚胎期第14.5天,转化生长因子βⅠ型和Ⅱ型受体在腺泡上皮和导管上皮内高表达,而腺体上皮细胞外的间充质没有表达.③p-ERK1/2主要也是表达在下颌下腺的上皮细胞中,与转化生长因子βⅠ型受体和Ⅱ型受体在下颌下腺中的表达基本一致.说明在小鼠下颌下腺的发育过程中,转化生长因子β蛋白可能通过与上皮细胞表面的受体结合,激活 ERK 信号通路来调节涎腺腺泡和导管的发育.
배경:소서적하합하선시연구타액선적발육적량호모형,전화생장인자β시기관발육화질병중중요적생장인자,단시재하합하선중전화생장인자β수체적표체이급작용궤제지금병불명학.목적:관찰배태소서하합하선발육과정중전화생장인자βⅠ형수체화Ⅱ형수체이급 p-ERK1/2적표체,게시전화생장인자β재소서연선발육중적작용.방법:취 C57BL/6J 소서배태기제14.5천적표본,사용전화생장인자βⅠ형수체화Ⅱ형수체이급p-ERK1/2항체,대소서적하합하선진행면역조화염색.취신생소서표본,대체관찰하합하선,병차사용소목정-이홍염색관찰기형태.결과여결론:①소서출생시,하합하선위우하합골하방;소목정-이홍염색발현소서하합하선적선포、도관화윤관세포야이경분화완성.②재배태기제14.5천,전화생장인자βⅠ형화Ⅱ형수체재선포상피화도관상피내고표체,이선체상피세포외적간충질몰유표체.③p-ERK1/2주요야시표체재하합하선적상피세포중,여전화생장인자βⅠ형수체화Ⅱ형수체재하합하선중적표체기본일치.설명재소서하합하선적발육과정중,전화생장인자β단백가능통과여상피세포표면적수체결합,격활 ERK 신호통로래조절연선선포화도관적발육.
BACKGROUND: The submandibular gland in mice is a typical study model for salivary development. Transforming growth factor beta is an essential growth factor during development and diseases. Til now the receptor of transforming growth factor beta and its function during salivary development has not been known wel . OBJECTIVE: To observe the expression of transforming growth factor beta typeⅠreceptor (Tgfbr1), transforming growth factor beta type Ⅱ receptor (Tgfbr2) and phospholated extracel ular signal-regulated kinase 1/2 in mouse submandibular glands in order to uncover the mechanism of transforming growth factor beta signaling in gland development. METHODS: Male and female C57BL/6J mice were mated to get embryos at embryonic day 14.5 (E14.5) and newborn stage. The samples were fixed in 4% paraformaldehyde and processed into paraffin embedded serial sections. The sections were stained with hematoxylin and eosin and immunohistochemistry by the antibodies of Tgfbr1, Tgfbr2 and phospholated extracel ular signal-regulated kinase 1/2 using standard procedures. RESULTS AND CONCLUSION: (1) At newborn stage, the submandibular gland was located under the mandible in mice. The submandibular gland alveolus, gland duct including intercalary duct developed wel . (2) At E14.5, Tgfbr1 and Tgfbr2 were expressed strongly in the submandibular glands epithelium, but not in the mesenchyme. (3) Expression of phospholated extracel ular signal-regulated kinase 1/2, similar to Tgfbr1 and Tgfbr2, was only detected in the epithelium. Therefore, transforming growth factor beta might regulate extracel ular signal-regulated kinase signaling in epithelial cells of the submandibular glands in order to control the submandibular gland development.