CAJ | 학술논문

组织构建%组织构建细胞学实验%脊髓全切%小胶质细胞%星形胶质细胞%少突胶质细胞细胞%墨西哥钝口螈%cd11b%胶质细胞原纤维酸性蛋白%髓鞘碱性蛋白%国家自然科学基金%组织构建图片文章組織構建%組織構建細胞學實驗%脊髓全切%小膠質細胞%星形膠質細胞%少突膠質細胞細胞%墨西哥鈍口螈%cd11b%膠質細胞原纖維痠性蛋白%髓鞘堿性蛋白%國傢自然科學基金%組織構建圖片文章
조직구건%조직구건세포학실험%척수전절%소효질세포%성형효질세포%소돌효질세포세포%묵서가둔구원%cd11b%효질세포원섬유산성단백%수초감성단백%국가자연과학기금%조직구건도편문장

背景:墨西哥钝口螈脊髓切断可以再生,再生过程伴随胶质细胞数目及分布的改变,研究墨西哥钝口螈脊髓全切后胶质细胞的变化,对进一步探讨其脊髓切断再生机制有重要意义.目的:观察墨西哥钝口螈脊髓全切后小胶质细胞、星形胶质细胞及少突胶质细胞的变化.方法:选用成年墨西哥钝口螈,分为脊髓全切组和对照组,利用免疫组织化学法观察脊髓全切后1,3和10 d 的损伤脊髓及周围区 cd11b 标记的小胶质细胞、胶质细胞原纤维酸性蛋白标记的星形胶质细胞及髓鞘碱性蛋白标记的少突胶质细胞的变化.结果与结论:脊髓全切后短期内 cd11b 染色阴性;脊髓损伤后胶质细胞原纤维酸性蛋白及髓鞘碱性蛋白阳性细胞染色强度,1 d 组阳性细胞染色强度与对照组比较无显著差异,3及10 d 组阳性细胞染色强度较对照组低.墨西哥钝口螈小胶质细胞染色阴性,可能存在不同于哺乳动物的标记蛋白;脊髓全切后3及10 d 在损伤脊髓及周围区的胶质细胞原纤维酸性蛋白及髓鞘碱性蛋白阳性细胞染色强度较对照组低,提示钝口螈脊髓急性损伤早期未见星形胶质细胞及少突胶质细胞增生,无胶质瘢痕形成.
배경:묵서가둔구원척수절단가이재생,재생과정반수효질세포수목급분포적개변,연구묵서가둔구원척수전절후효질세포적변화,대진일보탐토기척수절단재생궤제유중요의의.목적:관찰묵서가둔구원척수전절후소효질세포、성형효질세포급소돌효질세포적변화.방법:선용성년묵서가둔구원,분위척수전절조화대조조,이용면역조직화학법관찰척수전절후1,3화10 d 적손상척수급주위구 cd11b 표기적소효질세포、효질세포원섬유산성단백표기적성형효질세포급수초감성단백표기적소돌효질세포적변화.결과여결론:척수전절후단기내 cd11b 염색음성;척수손상후효질세포원섬유산성단백급수초감성단백양성세포염색강도,1 d 조양성세포염색강도여대조조비교무현저차이,3급10 d 조양성세포염색강도교대조조저.묵서가둔구원소효질세포염색음성,가능존재불동우포유동물적표기단백;척수전절후3급10 d 재손상척수급주위구적효질세포원섬유산성단백급수초감성단백양성세포염색강도교대조조저,제시둔구원척수급성손상조기미견성형효질세포급소돌효질세포증생,무효질반흔형성.
BACKGROUND: The spinal cord of Ambystoma mexicanum can regenerate after transection, and the number and distribution of gliacytes alter during regeneration. So it is important to observe the changes of gliacytes fol owing spinal cord transection in Ambystoma mexicanum in order to further discuss the mechanism of spinal cord regeneration. OBJECTIVE: To observe the changes of microglias, astrocytes and oligodendrocytes fol owing spinal cord transaction in Ambystoma mexicanum. METHODS: Adult Ambystoma mexicanum was selected and divided into spinal cord transection group and control group. Immunohistochemistry assay was performed to observe the changes in cd11b-labeled microglias, glial fibril ary acidic protein labeled astrocytes and myelin basic protein labeled oligodendrocytes in the injured region and peripheral region at 1, 3 and 10 days after spinal cord transection. RESULTS AND CONCLUSION: After transaction, the staining results of cd11b were negative. The staining intensity results of glial fibril ary acidic protein and myelin basic protein positive cells at 3 and 10 day were lower to the control group, but showed no significant difference from the control group at 1 day after transection. Microglias were negative in Ambystoma mexicanum, indicating the marker proteins of microglia in salamander may be different from those in the mammals. The staining intensity results of glial fibril ary acidic protein and myelin basic protein positive cells at 3 and 10 days were lower to the control group, which suggested that astrocytes and oligodendrocytes hyperplasia were not seen and no glial scar formed in early stage of spinal cord injury in Ambystoma mexicanum.