CAJ | 학술논문

背景:Ⅱ型胶原和硫酸软骨素是关节软骨的重要组成成分,不仅具有保护软骨的作用,还能抑制关节炎的病变.目的:通过检测类风湿因子和 C-反应蛋白的表达评价Ⅱ型胶原及Ⅱ型胶原复合硫酸软骨素注射对骨性关节炎的疗效.方法:采用改良 Hulth 法制作大鼠膝关节骨性关节炎模型,造模后模型组关节腔注射生理盐水、透明质酸钠组给予透明质酸钠凝胶注射、Ⅱ型胶原组给予单纯Ⅱ型胶原注射、Ⅱ型胶原复合硫酸软骨素组给予Ⅱ型胶原复合硫酸软骨素注射治疗.各组动物于术后进行日常观察、称质量、血清抗体及组织病理检查.结果与结论:造模后模型组大鼠血类风湿因子和 C-反应蛋白水平均有所升高,炎症加剧;而与模型组相比,各给药组大鼠类风湿因子和 C-反应蛋白水平均有所下降,炎症有所减轻,其中Ⅱ型胶原复合硫酸软骨素组大鼠血清中类风湿因子水平显著下降(P <0.01).结果显示Ⅱ型胶原与透明质酸钠均具有预防和治疗骨关节炎的作用,能缓解骨关节炎病变,Ⅱ型胶原联合硫酸软骨素的疗效更明显.
배경:Ⅱ형효원화류산연골소시관절연골적중요조성성분,불부구유보호연골적작용,환능억제관절염적병변.목적:통과검측류풍습인자화 C-반응단백적표체평개Ⅱ형효원급Ⅱ형효원복합류산연골소주사대골성관절염적료효.방법:채용개량 Hulth 법제작대서슬관절골성관절염모형,조모후모형조관절강주사생리염수、투명질산납조급여투명질산납응효주사、Ⅱ형효원조급여단순Ⅱ형효원주사、Ⅱ형효원복합류산연골소조급여Ⅱ형효원복합류산연골소주사치료.각조동물우술후진행일상관찰、칭질량、혈청항체급조직병리검사.결과여결론:조모후모형조대서혈류풍습인자화 C-반응단백수평균유소승고,염증가극;이여모형조상비,각급약조대서류풍습인자화 C-반응단백수평균유소하강,염증유소감경,기중Ⅱ형효원복합류산연골소조대서혈청중류풍습인자수평현저하강(P <0.01).결과현시Ⅱ형효원여투명질산납균구유예방화치료골관절염적작용,능완해골관절염병변,Ⅱ형효원연합류산연골소적료효경명현.
BACKGROUND: Type Ⅱ col agen and chondroitin sulfate are the major components of articular cartilage. They cannot only protect the cartilage, but also inhibit pathology of osteoarthritis. OBJECTIVE: To evaluate the curative effect of type Ⅱ col agen injection and type Ⅱ col agen-chondroitin sulfate composite injection on osteoarthritis by detecting the expression of rheumatoid factor and C-reactive protein. METHODS: A rat model of knee osteoarthritis was established by using improved Hulth’s method. The treatment was performed via injection into the articular cavity (treatment group A: type Ⅱ col agen injection;treatment group B: type Ⅱ col agen-chondroitin sulfate composite injection; positive control: sodium hyaluronate; model control: physiological saline). After operation, rats in each group were characterized with daily observation, weighing, serum antibody test and histological observation. RESULTS AND CONCLUSION: After treatment, the content of rheumatoid factor and C-reactive protein in rats’ serum of model group was increased, and inflammation was aggravated; while compared with the model group, the content of rheumatoid factor and C-reactive protein in the other groups, especial y in the treatment group B, were lower and the inflammation was eased (P < 0.01). The findings show that both type Ⅱ col agen and sodium hyaluronate can prevent and treat osteoarthritis effectively. The therapeutic effect of type Ⅱ col agen combined with chondroitin sulfate is better.