CAJ | 학술논문

生物材料%组织工程骨材料%磷酸钙骨水泥颗粒%聚甲基丙烯酸甲酯骨水泥颗粒%髓核细胞%细胞增殖%国家自然科学基金%生物材料图片文章生物材料%組織工程骨材料%燐痠鈣骨水泥顆粒%聚甲基丙烯痠甲酯骨水泥顆粒%髓覈細胞%細胞增殖%國傢自然科學基金%生物材料圖片文章
생물재료%조직공정골재료%린산개골수니과립%취갑기병희산갑지골수니과립%수핵세포%세포증식%국가자연과학기금%생물재료도편문장
biomaterials%tissue-engineered bone materials%calcium phosphate cement particles%polymethylmethacrylate particles%nucleus pulposus cells%cel proliferation%the National Natural Science Foundation of China%biomaterial photographs-containing paper

背景:椎体成形和椎体后凸成形治疗骨质疏松性椎体骨折过程中,常发生椎间盘骨水泥渗漏,但有关骨水泥进入椎间盘后对椎间盘细胞的影响尚不明确.目的:观察骨水泥颗粒对人髓核细胞增殖能力的影响.方法:采用体积分数0.001%,0.01%,0.1%,0.5%,1.0%的磷酸钙骨水泥颗粒或聚甲基丙烯酸甲酯骨水泥颗粒处理人正常髓核细胞,以常规培养的人正常髓核细胞为对照,以 CCK-8法检测各组细胞增殖活性.结果与结论:不同体积分数的磷酸钙骨水泥颗粒对人正常髓核细胞增殖无影响.体积分数0.001%,0.01%的聚甲基丙烯酸甲酯骨水泥颗粒对人正常髓核细胞增殖无显著影响;体积分数0.1%,0.5%,1.0%的聚甲基丙烯酸甲酯骨水泥颗粒明显抑制人正常髓核细胞的增殖,与对照组比较差异有显著性意义(P <0.05),且该效应具有时间依赖性和剂量依赖性:在处理3,6 d 时,对人正常髓核细胞增殖的抑制效应随颗粒体积分数的增加而逐渐增强;当颗粒体积分数为0.1%,0.5%和1.0%时,对人正常髓核细胞增殖抑制的效果随着时间的延长逐渐加强.
배경:추체성형화추체후철성형치료골질소송성추체골절과정중,상발생추간반골수니삼루,단유관골수니진입추간반후대추간반세포적영향상불명학.목적:관찰골수니과립대인수핵세포증식능력적영향.방법:채용체적분수0.001%,0.01%,0.1%,0.5%,1.0%적린산개골수니과립혹취갑기병희산갑지골수니과립처리인정상수핵세포,이상규배양적인정상수핵세포위대조,이 CCK-8법검측각조세포증식활성.결과여결론:불동체적분수적린산개골수니과립대인정상수핵세포증식무영향.체적분수0.001%,0.01%적취갑기병희산갑지골수니과립대인정상수핵세포증식무현저영향;체적분수0.1%,0.5%,1.0%적취갑기병희산갑지골수니과립명현억제인정상수핵세포적증식,여대조조비교차이유현저성의의(P <0.05),차해효응구유시간의뢰성화제량의뢰성:재처리3,6 d 시,대인정상수핵세포증식적억제효응수과립체적분수적증가이축점증강;당과립체적분수위0.1%,0.5%화1.0%시,대인정상수핵세포증식억제적효과수착시간적연장축점가강.
BACKGROUND: Vertebroplasty and kyphoplasty are widely used to treat osteoporotic vertebral fractures. Cement leakage into the intervertebral disc is one of common postoperative complications. The effect of cement leakage on intervertebral disc cells is not clear. OBJECTIVE: To investigate the influence of bone cement particles on the proliferative capacity of human nucleus pulposus cells.METHODS: Human nucleus pulposus cells were treated with bone cement particles including calcium phosphate cement or polymethylmethacrylate particles at the concentration of 0.001%, 0.01%, 0.1%, 0.5% and 1.0% (v/v). Routinely cultured human nucleus pulposus cells served as controls. Cel viability in different groups was tested with Cel Counting Kit-8. RESULTS AND CONCLUSION: Calcium phosphate cement particles of different concentrations had no significant effect on the proliferative capacity of human nucleus pulposus cells. Polymethylmethacrylate particles at the concentration of 0.001% and 0.01% (v/v) did not have a significant impact on the cel viability of normal human nucleus pulposus cells; but when the concentration was above 0.1% (v/v), polymethylmethacrylate particles could significantly inhibit the proliferation activity of the nucleus pulposus cells in a dose-dependent manner as compared with the control group (P < 0.05). After 3 or 6 days of treatment with the polymethylmethacrylate particles, the inhibition effect on human nucleus pulposus cells proliferation was increased gradual y with increasing concentration of the particles; when the polymethylmethacrylate particles were at the concentration of 0.1%, 0.5% and 1.0 % (v/v), the inhibition effect on the human nucleus pulposus cells proliferation was strengthened depending on the duration of particle treatment.