CAJ | 학술논문

背景:镁合金对成骨细胞的生物学行为及早期黏附是否有影响尚不明确.目的:分析镁合金 AZ31B 的表性,并评价其对成骨细胞黏附的影响.方法:采用扫描电镜及能谱分析明确镁合金 AZ31B 的表面形态及元素组成.以蛋白吸附实验检测镁合金 AZ31B 与钛合金对蛋白的吸附能力.将镁合金 AZ31B 与钛合金分别与小鼠前成骨细胞 MC3T3-El共培养于24孔板内,观察培养2,6,24 h 的细胞黏附情况.结果与结论:扫描电镜可见镁合金 AZ31B 表面较为粗糙,有利于细胞在其表面的黏附.能谱分析结果表明镁合金 AZ31B 的主要元素有镁、铝、锌,其中镁约占96%,铝约占3%,锌约占1%,另有一些其他元素,含量较少.镁合金 AZ31B 与钛合金的蛋白吸附率差异无显著性意义.培养2 h 时,镁合金 AZ31B 与钛合金上的细胞黏附率差异无显著性意义;培养6,24 h,镁合金 AZ31B 上的细胞黏附率显著低于钛合金上的细胞黏附率(P <0.01).培养于镁合金表面的成骨细胞贴壁展开,形态不规则,大多呈梭形,有较多突起,部分细胞间突起相互连接.表明镁合金 AZ31B 具有良好的细胞黏附性,适合于成骨细胞的早期黏附.
배경:미합금대성골세포적생물학행위급조기점부시부유영향상불명학.목적:분석미합금 AZ31B 적표성,병평개기대성골세포점부적영향.방법:채용소묘전경급능보분석명학미합금 AZ31B 적표면형태급원소조성.이단백흡부실험검측미합금 AZ31B 여태합금대단백적흡부능력.장미합금 AZ31B 여태합금분별여소서전성골세포 MC3T3-El공배양우24공판내,관찰배양2,6,24 h 적세포점부정황.결과여결론:소묘전경가견미합금 AZ31B 표면교위조조,유리우세포재기표면적점부.능보분석결과표명미합금 AZ31B 적주요원소유미、려、자,기중미약점96%,려약점3%,자약점1%,령유일사기타원소,함량교소.미합금 AZ31B 여태합금적단백흡부솔차이무현저성의의.배양2 h 시,미합금 AZ31B 여태합금상적세포점부솔차이무현저성의의;배양6,24 h,미합금 AZ31B 상적세포점부솔현저저우태합금상적세포점부솔(P <0.01).배양우미합금표면적성골세포첩벽전개,형태불규칙,대다정사형,유교다돌기,부분세포간돌기상호련접.표명미합금 AZ31B 구유량호적세포점부성,괄합우성골세포적조기점부.
BACKGROUND: Whether the biological behavior and early adhesion of osteoblasts can be affected by magnesium alloy is stil unknown. OBJECTIVE: To study surface properties of magnesium alloy AZ31B and to investigate the effects on the adhesion of osteoblasts in vitro. METHODS: Scanning electron microscope and energy dispersive spectrometer were used to study surface morphology and elemental composition of the magnesium alloy AZ31B. Protein absorption assay was applied to study the ability of magnesium alloy AZ31B and titanium alloy for protein adsorption. Then, mouse osteoblasts MC3T3-El were cocultured with magnesium alloy AZ31B or titanium alloy in 24-wel plates to observe the cel adhesion at 2, 6 and 24 hours. RESULTS AND CONCLUSION: The surface of AZ31B was rough which is beneficial for cel adhesion. Energy dispersive spectrometer results proved that the main elements of AZ31B were magnesium, aluminum, and zinc, among which, magnesium accounted for about 96%, aluminum for about 3%, and zinc for about 1%. There were also some other elements, but the content was little. Protein adsorption experiment results showed that AZ31B had good protein adsorption capacity. When the cells were cultured 2 hours, there was no significant difference between magnesium alloy AZ31B and titanium alloy in the rate of cel adhesion, but at 6 and 24 hour, the adhesion rate of magnesium alloy AZ31B was significantly lower than that of the titanium alloy (P < 0.01). The cells cultured on the surface of AZ31B expanded, and appeared with irregular shape, mostly fusiform, and more processes. Interconnected processes were seen among some cells. These show that the magnesium alloy AZ31B has a good capacity of cel adhesion, which is suitable for early osteoblast adhesion.