CAJ | 학술논문

生物材料%纳米生物材料%纳米纤维凝胶%脊髓损伤%神经干细胞%异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸%省级基金%生物材料图片文章生物材料%納米生物材料%納米纖維凝膠%脊髓損傷%神經榦細胞%異亮氨痠-賴氨痠-纈氨痠-丙氨痠-纈氨痠%省級基金%生物材料圖片文章
생물재료%납미생물재료%납미섬유응효%척수손상%신경간세포%이량안산-뢰안산-힐안산-병안산-힐안산%성급기금%생물재료도편문장
biomaterials%nanobiomaterials%nanofiber gel%spinal cord injury%neural stem cells%isoleucine-lysine-valine-alanine-valine%provincial grants-supported paper%biomaterial photographs-containing paper

背景:异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸具有良好的生物活性,在特定条件下可自组装成含IKVAV 纳米纤维凝胶支架,是一种天然的脊髓基质仿生材料.目的:进一步观察体外自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶对骨髓源性神经干细胞增殖及向神经元分化的影响.方法:取第2代新生 SD 大鼠骨髓源性神经干细胞与自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶共培养作为实验组,设置神经干细胞单独培养为对照组、单独自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶为空白对照组,CCK-8法及流式细胞仪检测培养1,3,5,7,10,14 d 的细胞增殖与神经干细胞向神经元分化的比例;MTT 法检测自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶对第2代新生 SD 大鼠骨髓源性神经干细胞的毒性.结果与结论:实验组不同时间点细胞增殖率及向神经元分化的细胞比例均高于对照组(P <0.05),两组细胞增殖均于第7天达峰值.MTT 法检测结果显示自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶无细胞毒性.表明异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶有较好的生物活性,可促进神经干细胞的增殖,提高神经干细胞向神经元分化的比例.
배경:이량안산-뢰안산-힐안산-병안산-힐안산구유량호적생물활성,재특정조건하가자조장성함IKVAV 납미섬유응효지가,시일충천연적척수기질방생재료.목적:진일보관찰체외자조장이량안산-뢰안산-힐안산-병안산-힐안산납미섬유응효대골수원성신경간세포증식급향신경원분화적영향.방법:취제2대신생 SD 대서골수원성신경간세포여자조장이량안산-뢰안산-힐안산-병안산-힐안산납미섬유응효공배양작위실험조,설치신경간세포단독배양위대조조、단독자조장이량안산-뢰안산-힐안산-병안산-힐안산납미섬유응효위공백대조조,CCK-8법급류식세포의검측배양1,3,5,7,10,14 d 적세포증식여신경간세포향신경원분화적비례;MTT 법검측자조장이량안산-뢰안산-힐안산-병안산-힐안산납미섬유응효대제2대신생 SD 대서골수원성신경간세포적독성.결과여결론:실험조불동시간점세포증식솔급향신경원분화적세포비례균고우대조조(P <0.05),량조세포증식균우제7천체봉치.MTT 법검측결과현시자조장이량안산-뢰안산-힐안산-병안산-힐안산납미섬유응효무세포독성.표명이량안산-뢰안산-힐안산-병안산-힐안산납미섬유응효유교호적생물활성,가촉진신경간세포적증식,제고신경간세포향신경원분화적비례.
BACKGROUND: Isoleucine-lysine-valine-alanine-valine has good biological activity, and under certain conditions, it can self-assemble into isoleucine-lysine-valine-alanine-valine nanofiber hydrogel scaffold which is a natural biomimetic material for the spinal cord matrix. OBJECTIVE: To observe the effects of self-assembled isoleucine-lysine-valine-alanine-valine nanofiber gel on proliferation and differentiation of bone marrow derived neural stem cells in vitro. METHODS: Passage 2 bone marrow derived neural stem cells were co-cultured with isoleucine-lysine-valine-alanine-valine as experimental group, neural stem cells cultured alone counted as control group, and isoleucine-lysine-valine-alanine-valine was set as blank control group. Cel proliferation rate and differentiation proportion of neural stem cells into neurons were measured at 1, 3, 5, 7, 10, 14 days after culture using Cel Counting Kit-8 and flow cytometry, respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay was used for detecting cytotoxicity of passage 2 bone marrow derived neural stem cells from new born Sprague-Dawley rats. RESULTS AND CONCLUSION: Cel proliferation rate and differentiation proportion of neural stem cells into neurons were significantly higher in the experimental group than in the control groups at each time (P < 0.05). Cel proliferation in the experimental and control groups peaked at day 7. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay detected no cytotoxicity of the isoleucine-lysine-valine-alanine-valine. Taken together, isoleucine-lysine-valine-alanine-valine nanofiber gel has a better biological activity, and can increase the differentiation proportion of neural stem cells into neurons.