CAJ | 학술논문

背景:部分文献报道壳聚糖对严重创伤的止血效果有限,因此以壳聚糖为基础止血剂的促凝血活性还有待进一步增强.目的:制备一种新型的壳聚糖/鞣花酸/红细胞膜脂复合海绵,评价其促凝血活性和细胞毒性.方法:通过冻干法制备壳聚糖海绵和壳聚糖乙酸盐海绵,然后再通过静电吸附法制备壳聚糖/鞣花酸/红细胞膜脂复合海绵.血浆复钙时间法观察3种海绵的促凝血活性,并检测3种海绵对 SD 大鼠肝脏的止血效果及对 L929细胞的毒性.结果与结论:壳聚糖/鞣花酸/红细胞膜脂复合海绵组的血浆复钙时间、出血时间、失血量均显著少于壳聚糖海绵组和壳聚糖乙酸盐海绵组(P <0.01).细胞实验显示壳聚糖/鞣花酸/红细胞膜脂复合海绵无细胞毒性.说明壳聚糖/鞣花酸/红细胞膜脂复合海绵具有良好的促凝血活性且无细胞毒性.
배경:부분문헌보도각취당대엄중창상적지혈효과유한,인차이각취당위기출지혈제적촉응혈활성환유대진일보증강.목적:제비일충신형적각취당/유화산/홍세포막지복합해면,평개기촉응혈활성화세포독성.방법:통과동간법제비각취당해면화각취당을산염해면,연후재통과정전흡부법제비각취당/유화산/홍세포막지복합해면.혈장복개시간법관찰3충해면적촉응혈활성,병검측3충해면대 SD 대서간장적지혈효과급대 L929세포적독성.결과여결론:각취당/유화산/홍세포막지복합해면조적혈장복개시간、출혈시간、실혈량균현저소우각취당해면조화각취당을산염해면조(P <0.01).세포실험현시각취당/유화산/홍세포막지복합해면무세포독성.설명각취당/유화산/홍세포막지복합해면구유량호적촉응혈활성차무세포독성.
BACKGROUND: Some previous studies have indicated that the hemostatic effect of chitosan is limited when dealing with severe injuries. Therefore, the procoagulant activity of chitosan-based hemostatic agents needs to be enhanced. OBJECTIVE: To prepare a novel chitosan/el agic acid/erythrocyte membrane lipid composite sponge and to evaluate its procoagulant activity and cytotoxicity.METHODS: Chitosan sponge and chitosan acetate sponge were prepared by freeze-drying method. Then chitosan/el agic acid/erythrocyte membrane lipid composite sponge was prepared by electrostatic adsorption method. Procoagulant activity of the chitosan, chitosan acetate, and chitosan/el agic acid/erythrocyte membrane lipid sponges was evaluated by the plasma recalcification time method. Hemostatic effect of these sponges was evaluated in the Sprague Dawley rat liver bleeding model, and the cytotoxicity to L929 cel line was evaluated. RESULTS AND CONCLUSION: The plasma recalcification time, bleeding time and blood loss of the chitosan/el agic acid/erythrocyte membrane lipid composite sponge group were significantly lower than those of the chitosan sponge and chitosan acetate sponge groups (P < 0.01). Cel culture experiment showed that the chitosan/el agic acid/erythrocyte membrane lipid composite sponge did not have cytotoxicity. The results indicate that the chitosan/el agic acid/erythrocyte membrane lipid composite sponge has the enhanced procoagulant activity and non-cytotoxicity.