中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
12期
109-114
,共6页
刘鉴峰%褚丽萍%王德芝%贺欣%徐宏艳%刘金剑
劉鑒峰%褚麗萍%王德芝%賀訢%徐宏豔%劉金劍
류감봉%저려평%왕덕지%하흔%서굉염%류금검
生物材料%材料生物相容性%聚酰胺-胺%乙酰化%细胞毒性%293T 细胞%增殖%细胞周期%国家自然科学基金%生物材料图片文章
生物材料%材料生物相容性%聚酰胺-胺%乙酰化%細胞毒性%293T 細胞%增殖%細胞週期%國傢自然科學基金%生物材料圖片文章
생물재료%재료생물상용성%취선알-알%을선화%세포독성%293T 세포%증식%세포주기%국가자연과학기금%생물재료도편문장
biomaterials%material biocompatibility%polyamidoamine%acetylation%cytotoxicity%293T cells%proliferation%cel cycle%the National Natural Science Foundation of China, biomaterial photographs-containing paper
背景:聚酰胺-胺型树枝状高分子纳米材料已被广泛应用于药物载体的研究,但由于整代聚酰胺-胺表面有大量带正电荷的氨基,具有一定的细胞毒性.目的:观察乙酰化对聚酰胺-胺细胞毒性的影响.方法:①细胞增殖检测:采用 MTT 法检测在含0,0.125,0.25,0.5,1,2,4μmol/L 乙酰化聚酰胺-胺的培养液中人胚肾293T 细胞的增殖.②细胞形态:倒置荧光显微镜观察在含4μmol/L 乙酰化聚酰胺-胺的培养液中人胚肾293T 细胞的形态.③细胞周期:流式细胞术检测在含0,5,10,15,20 mg/L 乙酰化聚酰胺-胺的培养液中人胚肾293T 细胞的细胞周期.结果与结论:聚酰胺-胺对293T 细胞具有一定的细胞毒性,在4μmol/L 浓度下48 h 的细胞存活率仅为52%,而乙酰化可显著降低聚酰胺-胺的细胞毒性(P <0.01);聚酰胺-胺孵育的细胞发生团缩,伸展性变差,而乙酰化聚酰胺-胺孵育的293T 细胞与正常培养细胞基本一致,具有良好的伸展性;乙酰化聚酰胺-胺对细胞周期无影响,而聚酰胺-胺在20 mg/L 较高质量浓度时可使细胞 S 期产生阻滞.表明乙酰化可以降低聚酰胺-胺的细胞毒性.
揹景:聚酰胺-胺型樹枝狀高分子納米材料已被廣汎應用于藥物載體的研究,但由于整代聚酰胺-胺錶麵有大量帶正電荷的氨基,具有一定的細胞毒性.目的:觀察乙酰化對聚酰胺-胺細胞毒性的影響.方法:①細胞增殖檢測:採用 MTT 法檢測在含0,0.125,0.25,0.5,1,2,4μmol/L 乙酰化聚酰胺-胺的培養液中人胚腎293T 細胞的增殖.②細胞形態:倒置熒光顯微鏡觀察在含4μmol/L 乙酰化聚酰胺-胺的培養液中人胚腎293T 細胞的形態.③細胞週期:流式細胞術檢測在含0,5,10,15,20 mg/L 乙酰化聚酰胺-胺的培養液中人胚腎293T 細胞的細胞週期.結果與結論:聚酰胺-胺對293T 細胞具有一定的細胞毒性,在4μmol/L 濃度下48 h 的細胞存活率僅為52%,而乙酰化可顯著降低聚酰胺-胺的細胞毒性(P <0.01);聚酰胺-胺孵育的細胞髮生糰縮,伸展性變差,而乙酰化聚酰胺-胺孵育的293T 細胞與正常培養細胞基本一緻,具有良好的伸展性;乙酰化聚酰胺-胺對細胞週期無影響,而聚酰胺-胺在20 mg/L 較高質量濃度時可使細胞 S 期產生阻滯.錶明乙酰化可以降低聚酰胺-胺的細胞毒性.
배경:취선알-알형수지상고분자납미재료이피엄범응용우약물재체적연구,단유우정대취선알-알표면유대량대정전하적안기,구유일정적세포독성.목적:관찰을선화대취선알-알세포독성적영향.방법:①세포증식검측:채용 MTT 법검측재함0,0.125,0.25,0.5,1,2,4μmol/L 을선화취선알-알적배양액중인배신293T 세포적증식.②세포형태:도치형광현미경관찰재함4μmol/L 을선화취선알-알적배양액중인배신293T 세포적형태.③세포주기:류식세포술검측재함0,5,10,15,20 mg/L 을선화취선알-알적배양액중인배신293T 세포적세포주기.결과여결론:취선알-알대293T 세포구유일정적세포독성,재4μmol/L 농도하48 h 적세포존활솔부위52%,이을선화가현저강저취선알-알적세포독성(P <0.01);취선알-알부육적세포발생단축,신전성변차,이을선화취선알-알부육적293T 세포여정상배양세포기본일치,구유량호적신전성;을선화취선알-알대세포주기무영향,이취선알-알재20 mg/L 교고질량농도시가사세포 S 기산생조체.표명을선화가이강저취선알-알적세포독성.
BACKGROUND: Polyamidoamine dendrimer nanomaterials have been widely used in drug carrier research, but there are many electropositive amino groups on the surface of the entire generation polyamidoamine, resulting in certain cytotoxicity.OBJECTIVE: To study the influence of acetylation on polyamidoamine cytotoxicity. METHODS: (1) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay: the cel proliferation of 293T cells incubated with acetylated polyamidoamine under 0, 0.125, 0.25, 0.5, 1, 2, 4 μmol/L concentrations was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. (2) Cel morphology: the cel morphology of 293T cells incubated with 4 μmol/L acetylated polyamidoamine was observed by inverted fluorescence microscope. (3) Cel cycle: the cel cycle of 293T cells incubated with acetylatedpolyamidoamine under 0, 5, 10, 15, 20 mg/L concentrations was detected by flow cytometry. RESULTS AND CONCLUSION: Polyamidoamine had cytotoxicity to 293T cells. The cel viability at 4 μmol/L concentration after 48 hours incubation was only 52%, and the acetylation could significantly decrease the cytotoxicity of polyamidoamine (P < 0.01). 293T cells incubated with polyamidoamine shrank and had bad stretching, while 293T cells incubated with acetylated polyamidoamine had good stretching. Acetylated polyamidoamine had no significant effect on the cel cycle, but polyamidoamine at 20 mg/L could block the cel cycle at S stage. Al the results show that acetylation can decrease the cytotoxicity of polyamidoamine.