医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2013年
10期
107-108
,共2页
吴峰萍张劼通讯%罗佐杰文静梁杏欢
吳峰萍張劼通訊%囉佐傑文靜樑杏歡
오봉평장할통신%라좌걸문정량행환
羟喜树碱%人肾上腺皮质癌SW-13细胞%细胞迁移
羥喜樹堿%人腎上腺皮質癌SW-13細胞%細胞遷移
간희수감%인신상선피질암SW-13세포%세포천이
hydroxycamptothecine%human adrenoeortical carcinoma SW-13%cels cel migration
目的:观察羟喜树碱对人肾上腺皮质癌SW-13细胞迁移的影响.方法:体外培养人肾上腺皮质癌SW-13细胞,取对数生长期细胞(同一代)进行实验,随机分为羟喜树碱干预组和对照组,干预组加入0.8ug/ml羟喜树碱.细胞划痕试验观察SW-13细胞的体外迁移力,分别在0h、48h拍照观察,应用Image pro plus 6软件处理分析结果.结果:1、光镜下可见羟喜树碱干预组SW-13细胞未迁移面积大于对照组;2、羟喜树碱干预组SW-13细胞的未迁移率大于对照组(P=0.000).结论:羟喜树碱有可能降低人肾上腺皮质癌SW-13细胞侵袭、迁移的能力.
目的:觀察羥喜樹堿對人腎上腺皮質癌SW-13細胞遷移的影響.方法:體外培養人腎上腺皮質癌SW-13細胞,取對數生長期細胞(同一代)進行實驗,隨機分為羥喜樹堿榦預組和對照組,榦預組加入0.8ug/ml羥喜樹堿.細胞劃痕試驗觀察SW-13細胞的體外遷移力,分彆在0h、48h拍照觀察,應用Image pro plus 6軟件處理分析結果.結果:1、光鏡下可見羥喜樹堿榦預組SW-13細胞未遷移麵積大于對照組;2、羥喜樹堿榦預組SW-13細胞的未遷移率大于對照組(P=0.000).結論:羥喜樹堿有可能降低人腎上腺皮質癌SW-13細胞侵襲、遷移的能力.
목적:관찰간희수감대인신상선피질암SW-13세포천이적영향.방법:체외배양인신상선피질암SW-13세포,취대수생장기세포(동일대)진행실험,수궤분위간희수감간예조화대조조,간예조가입0.8ug/ml간희수감.세포화흔시험관찰SW-13세포적체외천이력,분별재0h、48h박조관찰,응용Image pro plus 6연건처리분석결과.결과:1、광경하가견간희수감간예조SW-13세포미천이면적대우대조조;2、간희수감간예조SW-13세포적미천이솔대우대조조(P=0.000).결론:간희수감유가능강저인신상선피질암SW-13세포침습、천이적능력.
Objective To investigate the effects of HCPT on the migration of human adrenoeortical carcinoma cel line SW-13.Method SW-13 cel were culutured and the same cels were studied,which were randomly divided into there groops:The 0.8ug/mlHCPT intervention group and negative control group. The migration ability of SW-13 was measured by non-migration area.The size of the non-migration area was photographed at 0,48h and analyzed with the Image pro plus 6 software.Results At 48 hour, the non-migration area percentage in the experimental group were larger than negative Control .Conlusions HCPT could inhibit the migration ability of SW-13 cels. Objective To investigate the effects of HCPT on the migration of human adrenoeortical carcinoma cel line SW-13.Method SW-13 cel were culutured and the same cels were studied,which were randomly divided into there groops:The 0.8ug/mlHCPT intervention group and negative control group. The migration ability of SW-13 was measured by non-migration area.The size of the non-migration area was photographed at 0,48h and analyzed with the Image pro plus 6 software.Results At 48 hour, the non-migration area percentage in the experimental group were larger than negative Control .Conlusions HCPT could inhibit the migration ability of SW-13 cels.