CAJ | 학술논문

目的:通过检测>380MHz高频脉冲电磁场刺激下骨髓间充质干细中microRNA-370的表达变化规律,探讨mir-370对骨髓间充质干细胞可能的影响.方法:采用贴壁筛选法分离培养SD大鼠骨髓间充质干细胞,取第3代细胞随机分为两组:电磁场照射组、空白对照组,分别提取总RNA通过Realtime-PCR的结果推测mir-370对大鼠间充质干细胞中的bmp-7的调控作用.结果:经全骨髓细胞贴壁筛选法分离培养的SD大鼠骨髓间充质干细胞符合大鼠骨髓间充质干细胞基本特征.大鼠基因组模板Bmp7已扩增出来,大小与预测一致.在转染克隆有BMP7基因3’UTR质粒的实验组中,mimics组与空白组和NC组相比,(P>0.05)无统计学差异,证明mir-370不能与BMP7基因3’UTR结合,影响萤光素酶的活性.结论:mir-370不能负向调控bmp-7蛋白从而到达抑制骨髓间充质干细胞的作用.
목적:통과검측>380MHz고빈맥충전자장자격하골수간충질간세중microRNA-370적표체변화규률,탐토mir-370대골수간충질간세포가능적영향.방법:채용첩벽사선법분리배양SD대서골수간충질간세포,취제3대세포수궤분위량조:전자장조사조、공백대조조,분별제취총RNA통과Realtime-PCR적결과추측mir-370대대서간충질간세포중적bmp-7적조공작용.결과:경전골수세포첩벽사선법분리배양적SD대서골수간충질간세포부합대서골수간충질간세포기본특정.대서기인조모판Bmp7이확증출래,대소여예측일치.재전염극륭유BMP7기인3’UTR질립적실험조중,mimics조여공백조화NC조상비,(P>0.05)무통계학차이,증명mir-370불능여BMP7기인3’UTR결합,영향형광소매적활성.결론:mir-370불능부향조공bmp-7단백종이도체억제골수간충질간세포적작용.
Objective]:> 380MHz high-frequency pulsed electromagnetic fields(HF-PEMFs) stimulation by detecting bone marrow mesenchymal stem cels(BMSCs), microRNAs-370(mir-370) expression variation explore mir-370 on BMSCs may. [Methods]: adherence screening method is isolated and cultured SD rat BMSCs, the 3rd generation cels were randomly divided into two groups: the electromagnetic field radiation group, blank control group, respectively, total RNA was extracted by Realtime-PCR results speculate mir-370 regulation of bmp-7 in rat mesenchymal stem cels. [Results]: adherence screening method of whole bone marrow cels were isolated and cultured in SD rat BMSCs in line with the basic characteristics of rat BMSCs. The rat genome amplified template Bmp7 out, size and forecasts. Clone transfected the BMP7 gene 3'UTR plasmid experimental group Mimics the best choice group compared with the control group and the NC group, (P> 0.05) was not statisticaly different, and that mir-370 can not be combined with BMP7 gene 3'UTR affect the activity of the luciferase. [Conclusion]: mir-370 is not suppression of BMSCs to reach the negative regulation of bmp-7 protein.