CAJ | 학술논문

目的了解广西禽流感H5N1亚型病毒的基因特性.方法2011年在广西农贸市场采集污水、笼具涂抹、粪便标本,经H5亚型特异实时荧光定量PCR方法(Real-time fluorescence quantitative RT-PCR)检测,阳性样本进行病毒血凝素(hemagglutinin,HA)基因扩增后对产物直接测序,测序结果与已知参考毒株进行序列比对及系统进化分析.结果对阳性样本病毒HA基因测序获得6份HA序列,均分布在进化分支2.3.2的Ⅱ-1分支下.广西的6序列无论是氨基酸还是核苷酸的都是高度同源的,其核苷酸同源性在99.5%~100%,氨基酸同源性在99.5%~99.8%;序列测定的结果同时表明无论是受体特异性还是连接肽都是禽源的.结论2011年广西农贸市场流行的禽流感H5N1亚型病毒主要以进化分支2.3.2Ⅱ-1为主,均为禽源性的病毒.
목적료해엄서금류감H5N1아형병독적기인특성.방법2011년재엄서농무시장채집오수、롱구도말、분편표본,경H5아형특이실시형광정량PCR방법(Real-time fluorescence quantitative RT-PCR)검측,양성양본진행병독혈응소(hemagglutinin,HA)기인확증후대산물직접측서,측서결과여이지삼고독주진행서렬비대급계통진화분석.결과대양성양본병독HA기인측서획득6빈HA서렬,균분포재진화분지2.3.2적Ⅱ-1분지하.엄서적6서렬무론시안기산환시핵감산적도시고도동원적,기핵감산동원성재99.5%~100%,안기산동원성재99.5%~99.8%;서렬측정적결과동시표명무론시수체특이성환시련접태도시금원적.결론2011년엄서농무시장류행적금류감H5N1아형병독주요이진화분지2.3.2Ⅱ-1위주,균위금원성적병독.
@@@@Objective To elucidate the genetic characteristics of avian influenza H5N1 viruses in Guangxi, 2011.Methods Environmental samples collected from live animal markets in Guangxi were tested by real-time fluorescence quantitative RT-PCR method.The HA genes of H5N1 virus from positive samples were amplified by RT-PCR,and the fragments were sequenced directly.Both alignment and phylogenetic analysis were performed with sequences of the known reference strains.Results Six different HA sequences were obtained from the positive samples and belong to the distinst Clades 2.3.2 Ⅱ-1.The homology of genome ranged from 99.5% to 100% in nt and from 99.5% to 99.8% in aa,respectively. The sequence data also showed that the receptor specificity and the connecting peptide between HA1 and HA2 are still avian influenza origin.Conclusion The H5N1 subtype viruses obtained from live animal markets in 2011 were belong to Clade 2.3.2Ⅱ-1and were still avian influenza origin.