CAJ | 학술논문

采用里氏木霉为发酵菌种,通过液体深层发酵的方法,制备纤维素酶粗酶液,并用于酶促打浆.在温度30,℃、转速120,r/min 条件下培养96,h,系统研究了氮源和碳源种类、碳源用量、麸皮用量、微量元素液和营养元素液加入量对纤维素酶各组分酶活的影响.结果表明,在以0.2%硫酸铵为氮源,1%思茅松漂白硫酸盐浆为碳源,微量元素液和营养元素液加入量分别为0.2%、8%,麸皮加入量为1%的条件下,能够获得较高的纤维素酶酶活.酶促打浆研究结果表明:当纤维素酶用量为7,U/g 时,思茅松漂白硫酸盐浆的打浆度提高40%,且纸浆强度性能损失小.
채용리씨목매위발효균충,통과액체심층발효적방법,제비섬유소매조매액,병용우매촉타장.재온도30,℃、전속120,r/min 조건하배양96,h,계통연구료담원화탄원충류、탄원용량、부피용량、미량원소액화영양원소액가입량대섬유소매각조분매활적영향.결과표명,재이0.2%류산안위담원,1%사모송표백류산염장위탄원,미량원소액화영양원소액가입량분별위0.2%、8%,부피가입량위1%적조건하,능구획득교고적섬유소매매활.매촉타장연구결과표명:당섬유소매용량위7,U/g 시,사모송표백류산염장적타장도제고40%,차지장강도성능손실소.
The production technology of crude cellulase by using submerged fermentation with Trichoderma reesei and its application in the enzymatic beating of Simao Pine bleached kraft pulp were investigated in this study. Under the conditions of 30,℃ temperature,120,r/min rotating speed and 96,h culture time,the effects of different kinds of nitrogen and carbon sources,the dosage of carbon source,microelement liquid,bran and nutrition element liquid dosage on the activity of every component of the cellulase were studied. The results show that the optimum submerged fermentation conditions of Tricho-derma reesei for cellulase production were as follows:0.2% ammonium sulfate,1% Simao Pine bleached kraft pulp,0.2%microelement liquid,1% bran and 8% nutrition element liquid. In the enzymatic beating process,compared with the control pulp,the beating degree of the pulp treated with the enzyme dosage of 7,U/g could be increased by 40% and the physical properties of the pulp treated with enzyme were close to those of the control pulp.