CAJ | 학술논문

目的观察 Wnt5a 在 ALS 转基因鼠海马齿状回的表达变化,探讨 Wnt5a 在 ALS 转基因鼠发病中的作用机制.方法将30只 ALS 转基因鼠和同窝出生野生型鼠随机分两组,应用免疫荧光和 RT-PCR 技术分别于95d,108d,122d 龄检测 Wnt5a 在海马中表达情况.结果免疫荧光结果显示,与同窝出生的野生型鼠相比,在95d 变化不明显,108d,122d ALS 转基因鼠海马齿状回 Wnt5a 阳性细胞增多,且 Wnt5a 与 GFAP,β-tubulinⅢ共表达. RT-PCR 结果显示,与同窝野生型鼠比较,ALS 转基因鼠海马 Wnt5a mRNA 水平在95d 变化不明显,108d和122d 均升高(P <0.05).结论 Wnt5a 在 ALS 转基因鼠海马齿状回高表达,表明 Wnt5a 与肌萎缩脊髓侧索硬化症海马异常密切相关.
목적관찰 Wnt5a 재 ALS 전기인서해마치상회적표체변화,탐토 Wnt5a 재 ALS 전기인서발병중적작용궤제.방법장30지 ALS 전기인서화동와출생야생형서수궤분량조,응용면역형광화 RT-PCR 기술분별우95d,108d,122d 령검측 Wnt5a 재해마중표체정황.결과면역형광결과현시,여동와출생적야생형서상비,재95d 변화불명현,108d,122d ALS 전기인서해마치상회 Wnt5a 양성세포증다,차 Wnt5a 여 GFAP,β-tubulinⅢ공표체. RT-PCR 결과현시,여동와야생형서비교,ALS 전기인서해마 Wnt5a mRNA 수평재95d 변화불명현,108d화122d 균승고(P <0.05).결론 Wnt5a 재 ALS 전기인서해마치상회고표체,표명 Wnt5a 여기위축척수측색경화증해마이상밀절상관.
@@@@ Objective To study the expression of Wnt5a in dentate gyrus of adult amyotrophic lateral sclero -sis(ALS) transgenic mice,to explore the mechanism of Wnt5a in the pathogenesis of ALS transgenic mice .Methods Thirty ALS transgenic mice at the age of 95d,108d and 122d were randomly divided into two groups.They were detected with immunohistochemistry technique and RT -PCR.Results Compared with litter born-wild type mice,immunofluores-cence results showed that Wnt5a positive cells were increased significantly in dentate gyrus ALS transgenic mice at 108-day-old and 122-day-old.Wnt5a-labeled cell were co-localized with GFAP and β-tubulinⅢ.At 95-day-old,there were no significant differences.Compared with wild-type mice,Wnt5a RNA levels were significantly increased at 108d and 122d in the ALS transgenic mouse(P <0.05),but at 95-day-old,there were no significant differences.Conclusion The up-regulated expression of Wnt5a in the hippocampus of ALS transgenic mice suggests that Wnt 5a is related to amyotrophic lateral sclerosis.