CAJ | 학술논문

目的观察表皮生长因子(EGF)与 TGF-β1对小鼠成釉细胞金属基质蛋白酶-20(MMP20)基因表达的调控作用,并探讨其分子作用机制.方法利用 Real time-PCR 法分析 EGF,TGF-β1对成釉细胞 MMP20基因表达的影响;阻断 MAPK 通路,利用 Real time-PCR 法分析 EGF,TGF-β1对 MMP20基因表达的影响;利用双荧光素酶基因报告系统分析 EGF,TGF-β1对成釉细胞 MMP20基因启动子转录活性的影响.结果与对照组相比,EGF 与 TGF-β1均显著上调 MMP20基因表达;加入 MAPK 抑制剂后,显著抑制了 EGF 与 TGF-β1对 MMP20基因表达的作用.双荧光素酶报告基因检测系统显示:与对照组相比,EGF 与 TGF-β1显著促进 MMP20基因启动子的转录活性.结论 EGF 通过 P38通路与 ERK 通路上调 MMP20的表达;TGF-β1通过 p38通路与 JNK 通路上调 MMP20的表达.
목적관찰표피생장인자(EGF)여 TGF-β1대소서성유세포금속기질단백매-20(MMP20)기인표체적조공작용,병탐토기분자작용궤제.방법이용 Real time-PCR 법분석 EGF,TGF-β1대성유세포 MMP20기인표체적영향;조단 MAPK 통로,이용 Real time-PCR 법분석 EGF,TGF-β1대 MMP20기인표체적영향;이용쌍형광소매기인보고계통분석 EGF,TGF-β1대성유세포 MMP20기인계동자전록활성적영향.결과여대조조상비,EGF 여 TGF-β1균현저상조 MMP20기인표체;가입 MAPK 억제제후,현저억제료 EGF 여 TGF-β1대 MMP20기인표체적작용.쌍형광소매보고기인검측계통현시:여대조조상비,EGF 여 TGF-β1현저촉진 MMP20기인계동자적전록활성.결론 EGF 통과 P38통로여 ERK 통로상조 MMP20적표체;TGF-β1통과 p38통로여 JNK 통로상조 MMP20적표체.
@@@@Objective To investigate the regulatory effects of EGFandTGF -β1 induced matrix metalloproteinase20(MMP20) ex-pression in ameloblasts.Methods RT-PCR was used to observe the influence of EGF and TGF -β1 on MMP20 gene expression in amelo-blasts.Blocking MAPK path,RT-PCR was used to determinate the influence of EGF and TGF -β1 on MMP20 gene expression;Dual luciferase analysis was used to observe the effects of EGFandTGF -β1 on the transcriptional activity of MMP20 promoter.Results Compared with the control group,MMP20 expression was risen by EGF and TGF-β1.After joining MAPK inhibitor,MMP20 gene expression was significantly in -hibited.By the Dual-Luciferase Reporter Assay System,compared with the control group,the transcriptional activity of MMP20 which was stimulated by EGF and TGF-β1 had risen.Conclusion EGF enhanced MMP20 expression mediated by P38 and ERK signaling pathway in ameloblasts,and TGF-β1 enhanced MMP20 expression mediated by P38 and JNK signaling pathway in ameloblast .