CAJ | 학술논문

目的探讨心肌注射单宁酸(TA)对心肌梗死(MI)后心肌细胞外基质(ECM)重构的影响.方法建立大鼠心肌梗死模型,将60只大鼠随机分为单宁酸组(n =30),对照组(n =30).术后,用超声心动图评价心功能;术后4周,检测左、右心室重量指数,观察心肌梗死边缘区组织心肌细胞外基质(ECM)微结构;术后2周和4周,测定心肌组织胶原蛋白含量.结果符合实验要求的大鼠心梗模型成功率为70%左右;单宁酸组的心功能明显好于对照组(P <0.05);术后2周,单宁酸组的心梗区胶原蛋白含量高于对照组(56.75±6.78 vs 41.25±4.76,P <0.05);术后4周,单宁酸组左、右心室重量指数低于对照组(P <0.05);单宁酸心肌注射明显改善ECM 排列结构.结论在 MI 急性期,心肌注射 TA 可通过交联胶原,抑制胶原的降解,减轻心肌 ECM 重构.
목적탐토심기주사단저산(TA)대심기경사(MI)후심기세포외기질(ECM)중구적영향.방법건립대서심기경사모형,장60지대서수궤분위단저산조(n =30),대조조(n =30).술후,용초성심동도평개심공능;술후4주,검측좌、우심실중량지수,관찰심기경사변연구조직심기세포외기질(ECM)미결구;술후2주화4주,측정심기조직효원단백함량.결과부합실험요구적대서심경모형성공솔위70%좌우;단저산조적심공능명현호우대조조(P <0.05);술후2주,단저산조적심경구효원단백함량고우대조조(56.75±6.78 vs 41.25±4.76,P <0.05);술후4주,단저산조좌、우심실중량지수저우대조조(P <0.05);단저산심기주사명현개선ECM 배렬결구.결론재 MI 급성기,심기주사 TA 가통과교련효원,억제효원적강해,감경심기 ECM 중구.
@@@@Objective To investigate the effects of tannic acid(TA) on cardiac extracellular matrix(ECM) remodeling after acute myocardial infarction .Methods Myocardial infarction model in rats was induced by left coronary artery ligation.Sixty rats were enrolled and divided into tannic acid intramyocardial injection group (TA group,n =30) and saline solution intramyocardial injection group (control group,n =30).Heart function was evaluated by echocardio-graphy 24 hours after myocardial infarction and 2nd and 4th week after injection.Four weeks after injection,the structure of cardiac extracellular matrix ( ECM) in the infarcted borderline area was evaluated.Left ventricular weight index (LVWI) and right ventricular weight index (RVMI) were detected respectively.Collagen content in the infarcted area was evaluated by hydroxyproline colorimetry assay at 2 nd and 4 th week after injection.Results The arrangement of ECM was effectively improved and LVWI and RVMI was decreased in TA group after intramyocardial injection .Compared with control group,the collagen content was increased after intramyocardial injection of tannic acid at the early postinfarction stage(56.75mg/g ±6.78mg/g,41.25mg /g ±4.76mg /g,P <0.05).After injection,heart function of the rats was better in TA injection group than in control group.Conclusions Tannic acid after local intramyocardial injection prevents col -lagen matrix degradation via cross-linking fibrous collagen,and therefore,effectively attenuate ECM remodeling after AMI.