CAJ | 학술논문

目的探讨 p53基因突变在胃癌组织中的检测及临床意义.方法收集20例胃癌标本和25例胃良性疾病标本,分别提取其 DNA,用 PCR 法扩增 p53基因,基因测序后进行突变分析.结果20例胃癌患者中,p53基因的突变率为40.0%,25例胃良性疾病中,p53基因的突变率为8.0%(P <0.05).结论 p53基因突变在胃癌的发生过程中起重要作用,检测该基因的突变对胃癌的早期诊断、治疗及预后有重要临床意义.
목적탐토 p53기인돌변재위암조직중적검측급림상의의.방법수집20례위암표본화25례위량성질병표본,분별제취기 DNA,용 PCR 법확증 p53기인,기인측서후진행돌변분석.결과20례위암환자중,p53기인적돌변솔위40.0%,25례위량성질병중,p53기인적돌변솔위8.0%(P <0.05).결론 p53기인돌변재위암적발생과정중기중요작용,검측해기인적돌변대위암적조기진단、치료급예후유중요림상의의.
@@@@Objective To detect p53 gene mutation and evaluate its clinical significance .Methods A total of 20 cases of gastric cancer tissues and 25 cases of benign gastric disease were collected ,DNA was separately extracted from them .PCR was used to amplify p53 gene.After the gene sequencing,mutations were analyzed.Results The mutation rate of p53 in gastric cancer tissues was 40.0%,and the mutation rate of p53 in the gastric benign disease was 8.0%(P <0.05).Conclusion p53 gene mutation plays an important role during the process of gastric cancer,which has clinical significance .