CAJ | 학술논문

干细胞%干细胞移植%缺氧诱导因子1%脂肪干细胞%脂肪组织移植%基因转染%血管再生%质量保持率%省级基金%干细胞图片文章榦細胞%榦細胞移植%缺氧誘導因子1%脂肪榦細胞%脂肪組織移植%基因轉染%血管再生%質量保持率%省級基金%榦細胞圖片文章
간세포%간세포이식%결양유도인자1%지방간세포%지방조직이식%기인전염%혈관재생%질량보지솔%성급기금%간세포도편문장
stem cel s%stem cel transplantation%hypoxia-inducible factor-1%adipose-derived stem cel s%fat tissue transplantation%gene transfection%angiogenesis%mass maintenance rate%provincial grants-supported paper%stem cel photographs-containing paper

背景:自体颗粒脂肪组织作为理想的填充材料用于美容与重建修复领域,但因其移植后组织大量被吸收,严重影响了远期效果.目的:观察缺氧诱导因子1α基因转染脂肪干细胞对自体移植脂肪组织存活率的影响.方法:取健康成年人吸脂术后的脂肪组织分离脂肪干细胞并行原代及传代培养,传至第3代,调整细胞浓度为1×109 L-1,经缺氧诱导因子1α基因转染后调整细胞浓度为1×1011 L-1同一抽脂术后的脂肪组织颗粒并进行纯化,利用纤维蛋白胶的物理特性制备不同成分脂肪组织复合移植物,在20只裸鼠背部皮下随机分离3个腔隙,实验分为3组:基因修饰组移植经缺氧诱导因子1α基因修饰的脂肪干细胞+脂肪组织+纤维蛋白胶;基因未修饰组移植单纯脂肪干细胞+脂肪组织+纤维蛋白;生理盐水组移植生理盐水+脂肪组织+纤维蛋白.结果与结论:移植后3个月和6个月,各组移植物血管密度比较,基因修饰组>基因未修饰组>生理盐水组,组间比较差异有显著性意义(P<0.05);各组移植物脂肪细胞纤维坏死率比较,基因修饰组<基因未修饰组<生理盐水组,组间比较差异有显著性意义( P<0.05);各组移植物脂肪质量保持率比较,基因修饰组>基因未修饰组>生理盐水组,组间比较差异有显著性意义(P<0.05).结果证实,缺氧诱导因子1α基因转染脂肪干细胞可促进移植脂肪组织局部的血管再生,促进脂肪细胞的成活,增加脂肪组织的质量保持率,减少脂肪移植术后的纤维坏死程度.
배경:자체과립지방조직작위이상적전충재료용우미용여중건수복영역,단인기이식후조직대량피흡수,엄중영향료원기효과.목적:관찰결양유도인자1α기인전염지방간세포대자체이식지방조직존활솔적영향.방법:취건강성년인흡지술후적지방조직분리지방간세포병행원대급전대배양,전지제3대,조정세포농도위1×109 L-1,경결양유도인자1α기인전염후조정세포농도위1×1011 L-1동일추지술후적지방조직과립병진행순화,이용섬유단백효적물리특성제비불동성분지방조직복합이식물,재20지라서배부피하수궤분리3개강극,실험분위3조:기인수식조이식경결양유도인자1α기인수식적지방간세포+지방조직+섬유단백효;기인미수식조이식단순지방간세포+지방조직+섬유단백;생리염수조이식생리염수+지방조직+섬유단백.결과여결론:이식후3개월화6개월,각조이식물혈관밀도비교,기인수식조>기인미수식조>생리염수조,조간비교차이유현저성의의(P<0.05);각조이식물지방세포섬유배사솔비교,기인수식조<기인미수식조<생리염수조,조간비교차이유현저성의의( P<0.05);각조이식물지방질량보지솔비교,기인수식조>기인미수식조>생리염수조,조간비교차이유현저성의의(P<0.05).결과증실,결양유도인자1α기인전염지방간세포가촉진이식지방조직국부적혈관재생,촉진지방세포적성활,증가지방조직적질량보지솔,감소지방이식술후적섬유배사정도.
BACKGROUND:Autologous fat is an ideal fil ing material in aesthetic and reconstructive surgery. However, the long-term effects are poor because much tissue is absorbed after grafting. OBJECTIVE:To investigate the effects of hypoxia-inducible factor-1αgene transfected adipose-derived stem cel s on survival of autologous fat grafts. METHODS:Adipose-derived stem cel s were isolated from the subcutaneous adipose tissue of healthy adults after liposuction, primary cultured and sub-cultured. Passage 3 adipose-derived stem cel s at a density of 1× 109 cel s/L were transfected with hypoxia-inducible factor-1αgene. The cel s were then adjusted to 1×1011 Simultaneously, autologous fat from the same liposuction was selected and purified. The grafts were made according to physiological characteristics of fibrin glue. Three groups were designated. In the gene-modified group, hypoxia-inducible factor-1αgene-modified adipose-derived stem cel s, autologous fat+fibrin glue were transplanted into the back of nude mice. In the non-gene-modified group, adipose-derived stem cel s+autologous fat+fibrin glue were transplanted in the identical region. In the saline group, physiological saline+autologous fat+fibrin glue were transplanted. RESULTS AND CONCLUSION:At 3 and 6 months after transplantation, the vascular density in the graft was highest in the gene-modified group, fol owed by non-gene-modified group, and lastly the saline group and there was significant difference between groups (P<0.05). Fiber necrosis rate of adipose-derived stem cel s was lowest in the gene-modified group, fol owed by non-gene-modified group, and lastly the saline group and there was significant difference between groups (P<0.05). Fat maintenance rate was highest in the gene-modified group, fol owed by non-gene-modified group, and lastly the saline group and there was significant difference between groups (P<0.05). These findings suggest that hypoxia-inducible factor-1αgene transfected adipose-derived stem cel s can accelerate the vascularization in the local fat graft transplanted, promote the survival of adipose cel s, increase the mass maintenance rate of fat graft and lower the fibrosis necrosis degree after fat grafting.