CAJ | 학술논문

背景:不同的麻醉肿胀液成分及浓度对脂肪细胞及所获取的人脂肪干细胞的生物学特性是否存在毒性反应等负面影响,至今仍不得而知,国内外亦未见相关研究报道.目的:观察不同麻醉肿胀液对体外培养条件下人脂肪干细胞增殖与成脂分化的影响,以减少麻醉药物对其损害,提高人脂肪干细胞的利用率.方法:分离人脂肪干细胞并行原代及传代培养,取P3代细胞以终浓度为0.2 g/L的利多卡因、罗哌卡因和布比卡因麻醉肿胀液进行干预,并设立对照组.分析人脂肪干细胞受损及生长增殖情况并比较其成脂分化率.结果与结论:与对照组比较,各麻醉组每个时间点细胞上清液中乳酸脱氢酶值均增高,吸光度值降低,差异均有显著性意义(P <0.05);而布比卡因组与利多卡因组、罗哌卡因组比较,差异也有显著性意义(P <0.05).但各麻醉组人脂肪干细胞成脂分化率与对照组比较,差异无显著性意义(P >0.05).说明终浓度为0.2 g/L 的利多卡因、罗哌卡因和布比卡因麻醉肿胀液对人脂肪干细胞生长增殖有一定的抑制作用,但不影响其成脂分化能力.
배경:불동적마취종창액성분급농도대지방세포급소획취적인지방간세포적생물학특성시부존재독성반응등부면영향,지금잉불득이지,국내외역미견상관연구보도.목적:관찰불동마취종창액대체외배양조건하인지방간세포증식여성지분화적영향,이감소마취약물대기손해,제고인지방간세포적이용솔.방법:분리인지방간세포병행원대급전대배양,취P3대세포이종농도위0.2 g/L적리다잡인、라고잡인화포비잡인마취종창액진행간예,병설립대조조.분석인지방간세포수손급생장증식정황병비교기성지분화솔.결과여결론:여대조조비교,각마취조매개시간점세포상청액중유산탈경매치균증고,흡광도치강저,차이균유현저성의의(P <0.05);이포비잡인조여리다잡인조、라고잡인조비교,차이야유현저성의의(P <0.05).단각마취조인지방간세포성지분화솔여대조조비교,차이무현저성의의(P >0.05).설명종농도위0.2 g/L 적리다잡인、라고잡인화포비잡인마취종창액대인지방간세포생장증식유일정적억제작용,단불영향기성지분화능력.
BACKGROUND:Few reports have reported the effects of anesthetic with different ingredients and concentrations on the biological characteristics of adipocyte and human adipose-derived stem cel s, and the negative impacts such as toxic reaction are stil unclear. OBJECTIVE:To investigate the effects of different anesthetic conditions on the proliferation and adipogenic differentiation of human adipose-derived stem cel s in vitro in order to reduce anesthetic-induced damage and increase the availability of human adipose-derived stem cel s. METHODS:Human adipose-derived stem cel s were isolated from the subcutaneous adipose tissue of healthy adults after liposuction, and primary culture and subculture of human adipose-derived stem cel s were conducted. Passage 3 human adipose-derived stem cel s were treated with 0.2 g/L of final concentration medium which containing lidocaine, ropivacaine hydrochloride and bupivacaine, respectively. A control group was designated. The damage condition, growth and proliferation of human adipose-derived stem cel s were analyzed and the adipogenic differentiation ratio was compared. RESULTS AND CONCLUSION:Compared with the control group, the lactate dehydrogenase level of cel supernatants in lidocaine group, ropivacaine group and bupivacaine group at al time points was significantly increased, the absorbance value was decreased, and the difference was significant (P<0.05);furthermore, there was significant difference in the lactate dehydrogenase level of cel supernatants at al time points between lidocaine group and ropivacaine group or bupivacaine group (P<0.05). There was no significant difference of adipogenic differentiation ratio of human adipose-derived stem cel s between the four groups (P>0.05). The medium containing lidocaine, ropivacaine hydrochloride and bupivacaine at 0.2 g/L final concentration exhibits the growth and proliferation of human adipose-derived stem cel s, but it has no influence on the adipogenic differentiation of human adipose-derived stem cel s.