CAJ | 학술논문

干细胞%肿瘤干细胞%星形胶质瘤%脑%CD133%巢蛋白%神经元特异性烯醇化酶%胶质纤维酸性蛋白%细胞培养%免疫细胞化学%省级基金%干细胞图片文章榦細胞%腫瘤榦細胞%星形膠質瘤%腦%CD133%巢蛋白%神經元特異性烯醇化酶%膠質纖維痠性蛋白%細胞培養%免疫細胞化學%省級基金%榦細胞圖片文章
간세포%종류간세포%성형효질류%뇌%CD133%소단백%신경원특이성희순화매%효질섬유산성단백%세포배양%면역세포화학%성급기금%간세포도편문장
stem cel s%tumor stem cel s%astroglioma%brain%CD133%nestin%neuron specific enolase%glial fibrilary acidic protein%cel culture%immunocytochemistry%provincial grants-supported paper%stem cel photographs-containing paper

背景:肿瘤干细胞理论认为肿瘤中存在一小部分具有无限增殖潜能和自我更新能力,能够分化为成熟细胞表型的干细胞样细胞,对肿瘤发生、增殖、侵袭起关键作用.目的:建立体外分离、培养与鉴定星形胶质细胞瘤干细胞的方法.方法:采用直接培养法分离培养星形胶质细胞瘤干细胞.参照神经干细胞培养条件,进行体外培养.观察其增殖、分化并进行巢蛋白、CD133免疫细胞化学鉴定和诱导分化后神经元特异性烯醇化酶、胶质纤维酸性蛋白及O4免疫细胞化学鉴定.结果与结论:培养7-10 d,可形成大量悬浮生长巢蛋白及CD133免疫阳性的神经球,经诱导分化后细胞呈神经元特异性烯醇化酶、胶质纤维酸性蛋白或 O4免疫阳性.提示星形胶质细胞瘤中存在具有神经干细胞特性的肿瘤干细胞.CD133和巢蛋白是星形胶质细胞瘤干细胞重要的表面标记,可以用于星形胶质细胞瘤干细胞的分离.
배경:종류간세포이론인위종류중존재일소부분구유무한증식잠능화자아경신능력,능구분화위성숙세포표형적간세포양세포,대종류발생、증식、침습기관건작용.목적:건입체외분리、배양여감정성형효질세포류간세포적방법.방법:채용직접배양법분리배양성형효질세포류간세포.삼조신경간세포배양조건,진행체외배양.관찰기증식、분화병진행소단백、CD133면역세포화학감정화유도분화후신경원특이성희순화매、효질섬유산성단백급O4면역세포화학감정.결과여결론:배양7-10 d,가형성대량현부생장소단백급CD133면역양성적신경구,경유도분화후세포정신경원특이성희순화매、효질섬유산성단백혹 O4면역양성.제시성형효질세포류중존재구유신경간세포특성적종류간세포.CD133화소단백시성형효질세포류간세포중요적표면표기,가이용우성형효질세포류간세포적분리.
BACKGROUND:According to the theory of tumor stem cel s, there are a smal amount of stem cel-like cel s that exhibit infinite proliferative potential and self-renewal capacity, can differentiate into cel s with the phenotype of mature cel s and play a key role in tumor production, proliferation and invasion. OBJECTIVE:To investigate the feasibility of isolation, culture and identification of brain tumor stem cel s from human astrogliomas. METHODS:Brain tumor stem cel s were isolated by primary culture from human astrogliomas. These cel s were cultured under the culture condition of neural stem cel s. The clone spheres were identified with immunocytochemistery for nestin and CD133. At the same time, differentiated cel s were identified by immunocytochemistery for neuron specific enolase, glial fibril ary acidic protein and O4, respectively. RESULTS AND CONCLUSION:After 7-10 days of culture, a great number of neurospheres immunoreactive for nestin and CD133 were observed. After induced differentiation, these cel s were immunoreactive for neuron specific enolase, glial fibril ary acidic protein and O4. These findings suggest that there are brain tumor stem cel s with the characteristics of neural stem cel s in human astrogliomas. CD133 and nestin are key surface markers for brain tumor stem cel s, which can be used for isolation of brain tumor stem cel s.