中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
1期
86-91
,共6页
骆玉梅%范勇%陈欣洁%岳磊%黎青%余波澜%何文智%马晓燕%孙筱放
駱玉梅%範勇%陳訢潔%嶽磊%黎青%餘波瀾%何文智%馬曉燕%孫篠放
락옥매%범용%진흔길%악뢰%려청%여파란%하문지%마효연%손소방
干细胞%干细胞培养与分化%诱导性多能干细胞%绒毛细胞%产前诊断%自体移植%模型%国家自然科学基金%干细胞图片文章
榦細胞%榦細胞培養與分化%誘導性多能榦細胞%絨毛細胞%產前診斷%自體移植%模型%國傢自然科學基金%榦細胞圖片文章
간세포%간세포배양여분화%유도성다능간세포%융모세포%산전진단%자체이식%모형%국가자연과학기금%간세포도편문장
stem cel s%stem cel culture and differentiation%induced pluripotent stem cel s%vil us cel s%prenatal diagnosis%autologus transplantation%models%National Natural Science Foundation of China%stem cel photographs-containing paper
背景:诱导性多能干细胞因具有多能性特征,可以诱导分化为特定的细胞,包括神经细胞、造血细胞等.目的:建立产前诊断绒毛细胞来源的诱导性多能干细胞.方法:运用反转录病毒介导4种基因hOct4、hSox2、hc-Myc、hKlf4诱导产前诊断绒毛细胞,对建立的诱导性多能干细胞进行多能性、体内外分化能力、核型等鉴定.结果与结论:建立的诱导性多能干细胞能维持自我更新状态,在蛋白和mRNA水平上高表达全能性的标志基因,具有体内、外分化潜能;在体外长期培养能维持正常核型.说明4种全能性基因转入绒毛细胞可获得具有多能性的诱导性多能干细胞,这为胎儿的细胞自体移植治疗提供理想来源,为产前诊断疾病机制研究提供很好的细胞模型.
揹景:誘導性多能榦細胞因具有多能性特徵,可以誘導分化為特定的細胞,包括神經細胞、造血細胞等.目的:建立產前診斷絨毛細胞來源的誘導性多能榦細胞.方法:運用反轉錄病毒介導4種基因hOct4、hSox2、hc-Myc、hKlf4誘導產前診斷絨毛細胞,對建立的誘導性多能榦細胞進行多能性、體內外分化能力、覈型等鑒定.結果與結論:建立的誘導性多能榦細胞能維持自我更新狀態,在蛋白和mRNA水平上高錶達全能性的標誌基因,具有體內、外分化潛能;在體外長期培養能維持正常覈型.說明4種全能性基因轉入絨毛細胞可穫得具有多能性的誘導性多能榦細胞,這為胎兒的細胞自體移植治療提供理想來源,為產前診斷疾病機製研究提供很好的細胞模型.
배경:유도성다능간세포인구유다능성특정,가이유도분화위특정적세포,포괄신경세포、조혈세포등.목적:건립산전진단융모세포래원적유도성다능간세포.방법:운용반전록병독개도4충기인hOct4、hSox2、hc-Myc、hKlf4유도산전진단융모세포,대건립적유도성다능간세포진행다능성、체내외분화능력、핵형등감정.결과여결론:건립적유도성다능간세포능유지자아경신상태,재단백화mRNA수평상고표체전능성적표지기인,구유체내、외분화잠능;재체외장기배양능유지정상핵형.설명4충전능성기인전입융모세포가획득구유다능성적유도성다능간세포,저위태인적세포자체이식치료제공이상래원,위산전진단질병궤제연구제공흔호적세포모형.
@@@@ BACKGROUND:Due to pluripotent characteristics, induced pluripotent stem cel s can be induced to differentiate into the specific cel s, including nerve cel s and hematopoietic cel s. OBJECTIVE:To establish the induced pluripotent stem cel s generated from vil us cel s during prenatal diagnosis. METHODS:The prenatal diagnosis vil us cel s were induced by retroviral vectors encoding hOct4, hSox2, hKlf4 and hc-Myc. The pluripotency, differentiation ability and karyotype of the induced pluripotent stem cel s were detected. RESULTS AND CONCLUSION:The induced pluripotent stem cel s maintained the self-renewal ability with high pluripotent marker gene and protein expression and had in vivo and in vitro differentiation potential. The induced pluripotent stem cel s could maintain normal karyotype in vitro after long-term culture. The induced pluripotent stem cel s obtained from the prenatal diagnosis vil us cel s provide an ideal source for autologous cel-replacement therapy in the later life of the fetus, and can be used as a perfect model for the research of mechanism underlying prenatal y diagnosed diseases.
