CAJ | 학술논문

组织构建%组织构建基础实验%膀胱脱细胞基质%尿流改道%组织工程%膀胱肿瘤%上皮细胞%国家自然科学基金%组织构建图片文章組織構建%組織構建基礎實驗%膀胱脫細胞基質%尿流改道%組織工程%膀胱腫瘤%上皮細胞%國傢自然科學基金%組織構建圖片文章
조직구건%조직구건기출실험%방광탈세포기질%뇨류개도%조직공정%방광종류%상피세포%국가자연과학기금%조직구건도편문장
tissue construction%basic experiments of tissue construction%bladder acell ular matrix%urinary diversion%tissue engineering%bladder tumor%epithelial cells%National Natural Science Foundation of China%tissue construction photographs-containing paper

背景:根治性膀胱切除是目前对于肌层浸润性膀胱癌最有效的治疗手段,膀胱切除后尿流改道需利用肠道,并发症较多.目的:试用膀胱脱细胞基质和尿路上皮细胞构建组织工程化卷管进行尿流改道.方法:制作兔脱细胞基质,体外培养扩增兔膀胱上皮细胞,将上皮细胞种植到兔脱细胞基质上,培养7 d后制作成4 cm长的卷管;新西兰兔28只,其中实验组24只,分6组每组4只,分别行保留膀胱三角区的膀胱大部分切除,再将膀胱三角区与组织工程化卷管吻合,网膜包裹卷管;对照组4只,利用未种植细胞的脱细胞基质制作成流出道并与膀胱三角区吻合.分别于手术后1,2,4,8周取标本做苏木精-伊红染色,8周后对复合物进行免疫组织化学检测.结果与结论:实验组所有的动物均存活,苏木精-伊红染色显示流出道覆盖上皮,随着时间的推移,种植的上皮细胞与脱细胞基质逐渐融合,8周后免疫组织化学可见抗细胞角蛋白单克隆抗体 AE1/AE3阳性,抗ZO-1抗体阳性,证实流出道组织内有成熟上皮细胞覆盖,尿路上皮细胞之间有连接功能;对照组4只兔术后2周内均死亡,尸体解剖见流出道瘢痕形成,闭锁,双肾重度积水.结果表明,利用种植有尿路上皮细胞的脱细胞基质制作成组织工程化卷管在动物体内进行尿流改道是可行的,流出道内腔有成熟上皮覆盖,具有防止尿外渗的功能.
배경:근치성방광절제시목전대우기층침윤성방광암최유효적치료수단,방광절제후뇨류개도수이용장도,병발증교다.목적:시용방광탈세포기질화뇨로상피세포구건조직공정화권관진행뇨류개도.방법:제작토탈세포기질,체외배양확증토방광상피세포,장상피세포충식도토탈세포기질상,배양7 d후제작성4 cm장적권관;신서란토28지,기중실험조24지,분6조매조4지,분별행보류방광삼각구적방광대부분절제,재장방광삼각구여조직공정화권관문합,망막포과권관;대조조4지,이용미충식세포적탈세포기질제작성류출도병여방광삼각구문합.분별우수술후1,2,4,8주취표본주소목정-이홍염색,8주후대복합물진행면역조직화학검측.결과여결론:실험조소유적동물균존활,소목정-이홍염색현시류출도복개상피,수착시간적추이,충식적상피세포여탈세포기질축점융합,8주후면역조직화학가견항세포각단백단극륭항체 AE1/AE3양성,항ZO-1항체양성,증실류출도조직내유성숙상피세포복개,뇨로상피세포지간유련접공능;대조조4지토술후2주내균사망,시체해부견류출도반흔형성,폐쇄,쌍신중도적수.결과표명,이용충식유뇨로상피세포적탈세포기질제작성조직공정화권관재동물체내진행뇨류개도시가행적,류출도내강유성숙상피복개,구유방지뇨외삼적공능.
BACKGROUND:For muscle invasive bladder cancer, radical cystectomy is the most effective treatment now and urinary diversion is often necessary. The use of intestinal tissue for urinary diversion is frequently associated with complications. OBJECTIVE:To study the feasibility of tissue-engineered graft using bladder epithelial cel s and bladder acel ular matrix (BAM) for urinary diversion in rabbits. METHODS:BAM of rabbits was made in vitro. Bladder epithelial cel s of rabbits were cultivated and expanded in vitro, then implanted into the BAM and cultured for 7 days before making a 4 cm conduit. In the experimental group, 24 New Zealand rabbits, divided into six groups with four in each, were subjected to excising most of the bladder with bladder trigone retained. Then the retained bladder trigone were anatomized with the conduit which was then wrapped by omentum. In the control group, the conduit was made using unseeded BAM and anatomized with the retained bladder trigone in the four New Zealand rabbits. Specimens of tissue-engineered grafts were obtained at 1, 2, 4, and 8 weeks after surgery respectively and were stained with haematoxylin and eosin. Immunohistochemistry examination was done at 8 weeks postoperatively. RESULTS AND CONCLUSION:Al animals were alive in the experimental group. Hematoxylin and eosin staining showed epithelial coverage on the lumen of tissue-engineered conduit. With the time going on, grown epithelial cel s gradual y integrated with the BAM. Immunohistochemistry showed anti-cytokeratin AE1/AE3 antibody and anti-ZO1 antibody were positive, confirming that there were mature and functional epithelial cel s on the lumen of conduit. In the control group, four rabbits were al dead within 2 weeks. Scar formation, atresia, and severe hydronephrosis were found in the process of autopsy. It is feasible to prepare tissue-engineered grafts using bladder epithelial cel s and BAM for urinary diversion in rabbits.