中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
2期
205-209
,共5页
陈东%华文彬%叶树楠%杨述华%陈超%王小红%禹虔%刘先哲%索进平%聂磊
陳東%華文彬%葉樹楠%楊述華%陳超%王小紅%禹虔%劉先哲%索進平%聶磊
진동%화문빈%협수남%양술화%진초%왕소홍%우건%류선철%색진평%섭뢰
组织构建%骨组织构建%股骨头坏死%动物模型%乙醇%兔%X射线%MRI%国家自然科学基金%组织构建图片文章
組織構建%骨組織構建%股骨頭壞死%動物模型%乙醇%兔%X射線%MRI%國傢自然科學基金%組織構建圖片文章
조직구건%골조직구건%고골두배사%동물모형%을순%토%X사선%MRI%국가자연과학기금%조직구건도편문장
tissue construction%bone tissue construction%femoral head necrosis%animal models%ethanol%rabbits%X-ray%MRI%National Natural Science Foundation of China%tissue construction photographs-containing paper
背景:目前还难以建立合适的早期股骨头坏死的动物模型.目的:建立一个简单、标准和可靠的股骨头坏死动物模型用于实验研究.方法:X射线透视下将新西兰兔右侧股骨头中心钻孔并注入无水乙醇,左侧不做处理做对照.经过2,4和6周麻醉下处死动物获取股骨头.结果与结论:大体及X射线观察显示,造模侧股骨头第2周关节软骨颜色变暗、骨质密度不均匀;第6周,关节面有轻微凹陷,骨质低密度影较前进一步增大.MRI显示,造模侧股骨头第2周,T1加权股骨头负重区显示线样或不规则低信号,T2加权呈高信号;第6周,股骨头变性,软骨下骨折,关节面塌陷,新月体形成.对照侧第2,4,6周股骨头结果均常.组织病理学观察显示,造模侧股骨头第2周后,骨细胞核固缩、变性坏死.结果证实,2周后兔股骨头均发生了部分坏死,股骨头坏死的完整的外观形态,大体循环和关节软骨与人类早期股骨头坏死是相似的,提示实验建立了良好的股骨头坏死动物模型.
揹景:目前還難以建立閤適的早期股骨頭壞死的動物模型.目的:建立一箇簡單、標準和可靠的股骨頭壞死動物模型用于實驗研究.方法:X射線透視下將新西蘭兔右側股骨頭中心鑽孔併註入無水乙醇,左側不做處理做對照.經過2,4和6週痳醉下處死動物穫取股骨頭.結果與結論:大體及X射線觀察顯示,造模側股骨頭第2週關節軟骨顏色變暗、骨質密度不均勻;第6週,關節麵有輕微凹陷,骨質低密度影較前進一步增大.MRI顯示,造模側股骨頭第2週,T1加權股骨頭負重區顯示線樣或不規則低信號,T2加權呈高信號;第6週,股骨頭變性,軟骨下骨摺,關節麵塌陷,新月體形成.對照側第2,4,6週股骨頭結果均常.組織病理學觀察顯示,造模側股骨頭第2週後,骨細胞覈固縮、變性壞死.結果證實,2週後兔股骨頭均髮生瞭部分壞死,股骨頭壞死的完整的外觀形態,大體循環和關節軟骨與人類早期股骨頭壞死是相似的,提示實驗建立瞭良好的股骨頭壞死動物模型.
배경:목전환난이건립합괄적조기고골두배사적동물모형.목적:건립일개간단、표준화가고적고골두배사동물모형용우실험연구.방법:X사선투시하장신서란토우측고골두중심찬공병주입무수을순,좌측불주처리주대조.경과2,4화6주마취하처사동물획취고골두.결과여결론:대체급X사선관찰현시,조모측고골두제2주관절연골안색변암、골질밀도불균균;제6주,관절면유경미요함,골질저밀도영교전진일보증대.MRI현시,조모측고골두제2주,T1가권고골두부중구현시선양혹불규칙저신호,T2가권정고신호;제6주,고골두변성,연골하골절,관절면탑함,신월체형성.대조측제2,4,6주고골두결과균상.조직병이학관찰현시,조모측고골두제2주후,골세포핵고축、변성배사.결과증실,2주후토고골두균발생료부분배사,고골두배사적완정적외관형태,대체순배화관절연골여인류조기고골두배사시상사적,제시실험건립료량호적고골두배사동물모형.
BACKGROUND:Currently, it is difficult to prepare an animal model of early osteonecrosis of the femoral head (ONFH). OBJECTIVE:To develop a simple and dependable animal model of ONFH that al ows the evaluation of different therapeutic approaches. METHODS:Pure alcohol was injected into the centre of the right femoral head in New Zealand white rabbits under X-ray fluoroscopy, and the left femoral head with no treatment served as controls. After 2, 4 and 6 weeks, the animals were kil ed under anesthesia and the femoral heads were harvested. RESULTS AND CONCLUSION:Gross and X-ray observations indicated that the articular cartilage in the modeling side of the femoral head was dark in color and had the uneven bone density at the first two weeks after modeling. At 6 weeks, a slight depression was found on the articular surface, and the bone density shadow increased. MRI results showed that line or irregular T1-weighted signals and high T2-weighted signals were seen in the weight-bearing area of the femoral head at 2 weeks;femoral head degeneration, subchondral fracture, articular surface col apse, and crescent formation occurred at 6 weeks after modeling. In addition, there was no abnormality in the control side of the femoral head at 2, 4 and 6 weeks. Histopathological results showed karyopyknosis, degeneration and necrosis of the femoral head at 2 weeks after modeling. These findings verified that partial osteonecrosis was confirmed over a period of 2 weeks in al animals. The morphology of necrosis in combination with intact macrotexture and joint cartilage was similar to the features described in early ONFH in humans. We obtained a good efficacy and similarity animal model of the early stages of ONFH in humans. This model may be compatible to develop new therapeutic methods for ONFH.
