CAJ | 학술논문

组织构建%皮肤组织构建%超脉冲CO2激光%点阵激光%浅表性瘢痕%表皮干细胞%细胞角蛋白19%P63蛋白%省级基金%组织构建图片文章組織構建%皮膚組織構建%超脈遲CO2激光%點陣激光%淺錶性瘢痕%錶皮榦細胞%細胞角蛋白19%P63蛋白%省級基金%組織構建圖片文章
조직구건%피부조직구건%초맥충CO2격광%점진격광%천표성반흔%표피간세포%세포각단백19%P63단백%성급기금%조직구건도편문장
tissue construction%skin tissue construction%ultrapulse CO 2 laser%fractional laser%superficial scar%epidermal stem cel s%cytokeratin 19%P63 protein%provincial grants-supported paper%tissue construction photographs-containing paper

背景:临床上超脉冲CO2点阵激光治疗浅表性瘢痕的效果优良,但机制不清.目的:进一步验证超脉冲CO 2点阵激光干预兔耳浅表性瘢痕的效果,并探讨创面原位再生修复的相关机制.方法:用超脉冲CO 2点阵激光干预兔耳浅表性瘢痕,传统络合碘治疗和湿润烧伤膏治疗两种修复方法对创面进行修复.结果与结论:湿润烧伤膏组创面愈合显著短于络合碘组(P <0.01).超脉冲CO性瘢痕后即刻创缘无表皮干细胞标志分子细胞角蛋白19、P63蛋白表达,24 h后细胞角蛋白19、P63蛋白表达.湿润烧伤膏组治疗后第4天细胞角蛋白19、P63蛋白开始增多,第7天达到高峰,第14天逐渐减少;络合碘组治疗后第4天细胞角蛋白19、P63蛋白达高峰,第7天和第14天减少.湿润烧伤膏组细胞角蛋白19、P63蛋白强阳性表达率显著高于络合碘组(P<0.05).提示超脉冲CO2点阵激光结合湿润暴露疗法治疗兔耳浅表性瘢痕,能更好地启动和保护表皮干细胞,促进创面原位再生修复.2点阵激光干预兔耳浅表
배경:림상상초맥충CO2점진격광치료천표성반흔적효과우량,단궤제불청.목적:진일보험증초맥충CO 2점진격광간예토이천표성반흔적효과,병탐토창면원위재생수복적상관궤제.방법:용초맥충CO 2점진격광간예토이천표성반흔,전통락합전치료화습윤소상고치료량충수복방법대창면진행수복.결과여결론:습윤소상고조창면유합현저단우락합전조(P <0.01).초맥충CO성반흔후즉각창연무표피간세포표지분자세포각단백19、P63단백표체,24 h후세포각단백19、P63단백표체.습윤소상고조치료후제4천세포각단백19、P63단백개시증다,제7천체도고봉,제14천축점감소;락합전조치료후제4천세포각단백19、P63단백체고봉,제7천화제14천감소.습윤소상고조세포각단백19、P63단백강양성표체솔현저고우락합전조(P<0.05).제시초맥충CO2점진격광결합습윤폭로요법치료토이천표성반흔,능경호지계동화보호표피간세포,촉진창면원위재생수복.2점진격광간예토이천표
@@@@ BACKGROUND:The ultrapulse CO2 fractional laser has a good effect on the treatment of superficial scars, but the mechanism is unclear. OBJECTIVE:To investigate the effect of ultrapulse CO 2 fractional laser on the treatment of superficial scars in rabbit ear and to explore mechanism of wound in situ regeneration and repair. METHODS:The superficial scars in rabbit ear were induced with ultrapulse CO 2 fractional laser, then traditional iodophor treatment and moist exposed burn ointment treatment methods were used for repairing the wound. RESULTS AND CONCLUSION:The wound healing time of moist exposed burn ointment group was shorter than that of iodophor group (P<0.01). The epidermal stem cel s emerged after the superficial scars induced with ultrapulse CO2 fractional laser, and the expression of cytokeratin 19 and P63 protein was observed after induced for 24 hours. The number of cytokeratin 19 and P63 protein in the moist exposed burn ointment was increased after induced for 4 days and reached peak at 7 days and then decreased at 14 days. While the number of cytokeratin 19 and P63 protein in the iodophor group was increased after induced for 4 days, and then decreased after induced for 7 and 14 days. The expression of cytokeratin 19 and P63 protein in the moist exposed burn ointment group was significantly higher than that in the iodophor group (P<0.05). Ultrapulse CO2 combined with moist exposed burn ointment for the treatment of superficial scar in rabbit ear can better activate and protect the epidermal stem cel s, and promote wound repair and in situ regeneration. fractional laser