中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
2期
264-269
,共6页
张丽%陈娟%苏卫升%黄家君
張麗%陳娟%囌衛升%黃傢君
장려%진연%소위승%황가군
组织构建%皮肤组织构建%大豆异黄酮酊%创伤愈合%烫伤模型%小鼠%省级基金%组织构建图片文章
組織構建%皮膚組織構建%大豆異黃酮酊%創傷愈閤%燙傷模型%小鼠%省級基金%組織構建圖片文章
조직구건%피부조직구건%대두이황동정%창상유합%탕상모형%소서%성급기금%조직구건도편문장
tissue construction%skin tissue construction%soy isoflavone tincture%wound healing%scald models%mice%provincial grants-supported paper%tissue construction photographs-containing paper
背景:雌激素具有促进创面愈合的作用,大豆异黄酮能与雌激素受体结合,具有明显的雌激素样效应.目的:探索大豆异黄酮酊的制备、观察其对小鼠烫伤创面愈合的影响.方法:用冷浸渍法制备大豆异黄酮酊,筛选最佳有效浓度.用昆明小鼠造Ⅱ度烫伤模型,随机分为6组.药物组分别应用0.100,0.361,1.000,3.610 g/L的大豆异黄酮酊局部涂抹;溶剂对照组应用体积分数75%乙醇局部涂抹;空白对照组不作任何处理.从第3天起,隔天记录创面面积、计算未愈合创面占原始创面的百分比,于造模后第3,9,14天取创面组织做病理切片,观察形态学改变和创面愈合情况.结果与结论:大豆异黄酮酊的最佳效应浓度是3.61 g/mL.在创伤后第5,7,9,11,13天,大豆异黄酮酊组的未愈合创面占原始创面的百分比明显小于溶剂对照组和空白组(P <0.05).病理组织切片显示,第9天,大豆异黄酮酊组的成纤维细胞明显多于溶剂对照组和空白组,第14天,大豆异黄酮酊组的表皮生长情况比溶剂对照组和空白组好.表明以75%乙醇为溶剂制备大豆异黄酮酊是可行的;3.61 g/L的大豆异黄酮酊对小鼠创面的愈合具有明显的促进用.
揹景:雌激素具有促進創麵愈閤的作用,大豆異黃酮能與雌激素受體結閤,具有明顯的雌激素樣效應.目的:探索大豆異黃酮酊的製備、觀察其對小鼠燙傷創麵愈閤的影響.方法:用冷浸漬法製備大豆異黃酮酊,篩選最佳有效濃度.用昆明小鼠造Ⅱ度燙傷模型,隨機分為6組.藥物組分彆應用0.100,0.361,1.000,3.610 g/L的大豆異黃酮酊跼部塗抹;溶劑對照組應用體積分數75%乙醇跼部塗抹;空白對照組不作任何處理.從第3天起,隔天記錄創麵麵積、計算未愈閤創麵佔原始創麵的百分比,于造模後第3,9,14天取創麵組織做病理切片,觀察形態學改變和創麵愈閤情況.結果與結論:大豆異黃酮酊的最佳效應濃度是3.61 g/mL.在創傷後第5,7,9,11,13天,大豆異黃酮酊組的未愈閤創麵佔原始創麵的百分比明顯小于溶劑對照組和空白組(P <0.05).病理組織切片顯示,第9天,大豆異黃酮酊組的成纖維細胞明顯多于溶劑對照組和空白組,第14天,大豆異黃酮酊組的錶皮生長情況比溶劑對照組和空白組好.錶明以75%乙醇為溶劑製備大豆異黃酮酊是可行的;3.61 g/L的大豆異黃酮酊對小鼠創麵的愈閤具有明顯的促進用.
배경:자격소구유촉진창면유합적작용,대두이황동능여자격소수체결합,구유명현적자격소양효응.목적:탐색대두이황동정적제비、관찰기대소서탕상창면유합적영향.방법:용랭침지법제비대두이황동정,사선최가유효농도.용곤명소서조Ⅱ도탕상모형,수궤분위6조.약물조분별응용0.100,0.361,1.000,3.610 g/L적대두이황동정국부도말;용제대조조응용체적분수75%을순국부도말;공백대조조불작임하처리.종제3천기,격천기록창면면적、계산미유합창면점원시창면적백분비,우조모후제3,9,14천취창면조직주병리절편,관찰형태학개변화창면유합정황.결과여결론:대두이황동정적최가효응농도시3.61 g/mL.재창상후제5,7,9,11,13천,대두이황동정조적미유합창면점원시창면적백분비명현소우용제대조조화공백조(P <0.05).병리조직절편현시,제9천,대두이황동정조적성섬유세포명현다우용제대조조화공백조,제14천,대두이황동정조적표피생장정황비용제대조조화공백조호.표명이75%을순위용제제비대두이황동정시가행적;3.61 g/L적대두이황동정대소서창면적유합구유명현적촉진용.
BACKGROUND:Estrogen has a role to promote wound healing, and combination of soy isoflavones and estrogen receptor presents an obvious estrogen-like effect. OBJECTIVE:To explore the preparation of soy isoflavone tincture and to study its influence on wound healing in scalded mice. METHODS:Soy isoflavone tincture was prepared by cold dipping and the most effective concentration was screened. At the beginning of the experiment, al of the mice were used to prepare deep partial-thickness scald models and then randomly divided into soy isoflavone tincture (0.100, 0.361, 1.000, 3.610 g/L), solvent control (75%ethanol) and blank (without treatment) groups. From the 3rd day on, nonhealing rates of wound were assessed every other day. Simultaneously, the histological and morphological changes were observed and analyzed at days 3, 9, 14 after modeling. RESULTS AND CONCLUSION:The most effective concentration of soybean isoflavone tincture was 3.61 g/L. On days 5, 7, 9, 11, 13 after injury, the nonhealing rate of wound in the soybean isoflavone tincture group was significantly less than that of the blank group (P<0.05), as wel as that of the solvent control group (P<0.05). On day 9 post scalded, the histomorphology displayed that there were many more fibroblasts in the granulation tissue in the soy isoflavone tincture group than those of the blank group, also than those of the solvent control group. On day 14 post scalded, the histomorphology showed that the epithelial layer in the soy isoflavone tincture group was thicker than that of the other two groups. It is a viable method that soy isoflavone tincture is prepared in 75%ethanol solution, and its concentration of 3.61 g/L can significantly accelerate the wound healing in scalded mice by local application.