CAJ | 학술논문

背景:建立骨性关节炎的中医证候模型,有利于对其进行辨证施治研究.目的:采用病、证结合的方法建立肾虚血瘀型膝骨关节炎大鼠模型.方法:选择2月龄雌性SPF 级SD大鼠30只,随机分为正常组、膝骨关节炎组和复合模型组,每组10只.采用经典的 Hulth 造模法建立膝骨关节炎模型,去卵巢法建立肾虚模型,肾上腺皮质激素和肾上腺素法建立血瘀模型.复合模型由上述3种模型复合而成,先建立肾虚模型,8周后同时进行血瘀模型与骨关节炎模型造模.结果与结论:复合模型组大鼠在肾虚模型建立后第3周时出现精神萎靡、活动不灵活、便溏等表现,在血瘀模型建立后第6周时大鼠出现皮毛无光泽、喜背光、舌质瘀紫、尾色及爪甲瘀青,体质量下降;低切还原黏度增高,血小板聚集性增强等血流缓慢、循环障碍特点;组织学染色观察发现膝骨关节炎组关节软骨结构有一定破坏但轻于复合模型组.可见实验成功建立了大鼠肾虚血瘀型大鼠膝骨关节炎模型,而肾虚血瘀加重了膝骨关节软骨的退变.
배경:건립골성관절염적중의증후모형,유리우대기진행변증시치연구.목적:채용병、증결합적방법건립신허혈어형슬골관절염대서모형.방법:선택2월령자성SPF 급SD대서30지,수궤분위정상조、슬골관절염조화복합모형조,매조10지.채용경전적 Hulth 조모법건립슬골관절염모형,거란소법건립신허모형,신상선피질격소화신상선소법건립혈어모형.복합모형유상술3충모형복합이성,선건립신허모형,8주후동시진행혈어모형여골관절염모형조모.결과여결론:복합모형조대서재신허모형건립후제3주시출현정신위미、활동불령활、편당등표현,재혈어모형건립후제6주시대서출현피모무광택、희배광、설질어자、미색급조갑어청,체질량하강;저절환원점도증고,혈소판취집성증강등혈류완만、순배장애특점;조직학염색관찰발현슬골관절염조관절연골결구유일정파배단경우복합모형조.가견실험성공건립료대서신허혈어형대서슬골관절염모형,이신허혈어가중료슬골관절연골적퇴변.
@@@@ BACKGROUND:To establish a model of osteoarthritis with traditional Chinese medicine syndromes is conductive to the syndrome differentiation studies. OBJECTIVE:To establish a rat model of knee osteoarthritis with kidney deficiency and blood stagnation. METHODS:A total of 30 female Sprague-Dawley rats aged 2 months, SPF grade, were randomly divided into normal group, knee osteoarthritis group and knee osteoarthritis with kidney deficiency and bloods stagnation group (combined group), 10 rats in each group. Knee osteoarthritis models were established using Hulth method, kidney deficiency models were established using ovariectomized method, and blood stagnation models were established by injection of adrenal corticosteroids and epinephrine. Combined models were prepared based on the above-mentioned three models. Kidney deficiency models were prepared first fol owed by establishment of knee osteoarthritis and bloods stagnation models. RESULTS AND CONCLUSION:Rats in the combined group presented listlessness, non-flexible activities and loose stools at 3 weeks after establishment of kidney deficiency models, and coat lackluster, ptosis, obvious weight loss and blue tail at 6 weeks after establishment of bloods stagnation models. Rats in the combined group also showed that the reducing viscosity was significantly up-regulated and the platelet aggregation reinforced, with blood flow slow-moving, circulatory disorder and so on. Meanwhile, the articular cartilage of the knee osteoarthritis group showed cataplasia, but it was slighter compared with the combined group. The rat model of knee osteoarthritis with kidney deficiency and blood stagnation is established successful y, and kidney deficiency and blood stagnation can aggravate articular cartilage degeneration.