CAJ | 학술논문

背景:中药及其提取物在改善皮肤组织结构、提高抗氧化酶活性、抑制基质金属蛋白酶高表达、增强皮肤免疫防御功能等多方面对皮肤光老化起着防治作用.目的:验证丹酚酸B对皮肤光老化的保护作用.方法:同时建立小鼠皮肤体外细胞培养紫外线损伤模型及小鼠紫外照射致皮肤衰老模型,给予不同剂量的丹酚酸B,通过 MTT法,单细胞凝胶电泳等观察各组皮肤细胞损伤程度以及丹酚酸B对细胞损伤的保护作用,小鼠灌胃口服不同剂量丹酚酸 B,通过对皮肤组织羟脯氨酸、超氧化物歧化酶等含量的检测,评价丹酚酸B是否具有抑制皮肤光老化的作用.结果与结论:紫外照射可使皮肤丙二醛含量明显上升,羟脯氨酸含量,超氧化物歧化酶和谷胱甘肽氧化酶活性下降.丹酚酸B能有效地对紫外线引起细胞致死性损伤起到保护作用,能显著降低皮肤组织丙二醛含量,提高羟脯氨酸含量,超氧化物歧化酶和谷胱甘肽氧化酶活性而起到抑制皮肤光老化作用.结果证实丹酚酸B具有保护皮肤光老化作用.
배경:중약급기제취물재개선피부조직결구、제고항양화매활성、억제기질금속단백매고표체、증강피부면역방어공능등다방면대피부광노화기착방치작용.목적:험증단분산B대피부광노화적보호작용.방법:동시건립소서피부체외세포배양자외선손상모형급소서자외조사치피부쇠로모형,급여불동제량적단분산B,통과 MTT법,단세포응효전영등관찰각조피부세포손상정도이급단분산B대세포손상적보호작용,소서관위구복불동제량단분산 B,통과대피부조직간포안산、초양화물기화매등함량적검측,평개단분산B시부구유억제피부광노화적작용.결과여결론:자외조사가사피부병이철함량명현상승,간포안산함량,초양화물기화매화곡광감태양화매활성하강.단분산B능유효지대자외선인기세포치사성손상기도보호작용,능현저강저피부조직병이철함량,제고간포안산함량,초양화물기화매화곡광감태양화매활성이기도억제피부광노화작용.결과증실단분산B구유보호피부광노화작용.
BACKGROUND:Chinese medicine and its extract can improve skin structure, improve the activity of antioxidant enzymes, inhibit high expression of matrix metal oproteinase, and enhance the immune defense function of the skin, which plays a preventive effect against skin photoaging. OBJECTIVE:To observe the protective effect of salvianolic acid B on skin photoaging in mice. METHODS:Mouse skin cel s were cultured and irradiated in vitro to prepare UV damage models, which were treated with different doses of salvianolic acid B. MTT and single cel electrophoresis were performed to observe the severity of skin injury and protective role of salvianolic acid B on cel injury. Skin photoaging models were prepared through UV irradiation in mice, and then model mice were intragastrical y administrated with different doses of salvianolic acid B. Hydroxyproline and superoxide dismutase contents were determined to evaluate whether salvianolic acid B can inhibit skin photoaging. RESULTS AND CONCLUSION:UV irradiation could increase malondialdehyde content in the skin, decrease hydroxyproline content as wel as superoxide dismutase and glutathione oxidase activity. Salvianolic acid B could effectively protect the cel s from UV damage, remarkably reduce malondialdehyde content in the skin, increase hydroxyproline, superoxide dismutase and glutathione oxidase contents, playing an inhibitory effect on skin photoaging. These findings indicate that salvianolic acid B has a protective effect on skin photoaging in mice.