CAJ | 학술논문

背景:在皮肤中受体蛋白酪氨酸磷酸酶kappa的调控至关重要,而转化生长因子β似乎是其调控的上游因子,既然Notch信号和转化生长因子β信号通道如此相关,那么Notch是不是也参加了转化生长因子β信号对受体蛋白酪氨酸磷酸酶kappa的调控呢?目的:探讨 Notch 信号通道在人角质形成细胞中对转化生长因子β调控受体蛋白酪氨酸磷酸酶 kappa的作用的影响.方法:在分别用Jagged-1激活和用Γ-分泌酶抑制剂抑制Notch信号通道后,加入转化生长因子β,同时设立对照组,用Real-time PCR测试人角质形成细胞中受体蛋白酪氨酸磷酸酶kappa mRNA表达量.结果与结论:覆盖率为40%的角质形成细胞在加入了转化生长因子β后,受体蛋白酪氨酸磷酸酶kappa mRNA 量在各时间点均高于对照组.在用 Jagged-1激活 Notch 通道的角质形成细胞中,单独加入Jagged-1、转化生长因子β及两者都加入时均高于对照组(P<0.05,P<0.01).在用γ-分泌酶抑制剂抑制Notch通道的角质形成细胞中,只加入转化生长因子β显著高于对照组(P<0.01),只加入γ-分泌酶抑制剂和两者均加入时与对照组比较,差异无显著性意义(P >0.05).说明加入转化生长因子β导致角质形成细胞中受体蛋白酪氨酸磷酸酶kappa表达增加,而分别对Notch信号进行激活和抑制后发现,受体蛋白酪氨酸磷酸酶kappa信号分别显著增加和显著被抑制.所以在转化生长因子β升高受体蛋白酪氨酸磷酸酶kappa表达过程中Notch信号通道是非常重要且不可或缺的.
배경:재피부중수체단백락안산린산매kappa적조공지관중요,이전화생장인자β사호시기조공적상유인자,기연Notch신호화전화생장인자β신호통도여차상관,나요Notch시불시야삼가료전화생장인자β신호대수체단백락안산린산매kappa적조공니?목적:탐토 Notch 신호통도재인각질형성세포중대전화생장인자β조공수체단백락안산린산매 kappa적작용적영향.방법:재분별용Jagged-1격활화용Γ-분비매억제제억제Notch신호통도후,가입전화생장인자β,동시설립대조조,용Real-time PCR측시인각질형성세포중수체단백락안산린산매kappa mRNA표체량.결과여결론:복개솔위40%적각질형성세포재가입료전화생장인자β후,수체단백락안산린산매kappa mRNA 량재각시간점균고우대조조.재용 Jagged-1격활 Notch 통도적각질형성세포중,단독가입Jagged-1、전화생장인자β급량자도가입시균고우대조조(P<0.05,P<0.01).재용γ-분비매억제제억제Notch통도적각질형성세포중,지가입전화생장인자β현저고우대조조(P<0.01),지가입γ-분비매억제제화량자균가입시여대조조비교,차이무현저성의의(P >0.05).설명가입전화생장인자β도치각질형성세포중수체단백락안산린산매kappa표체증가,이분별대Notch신호진행격활화억제후발현,수체단백락안산린산매kappa신호분별현저증가화현저피억제.소이재전화생장인자β승고수체단백락안산린산매kappa표체과정중Notch신호통도시비상중요차불가혹결적.
BACKGROUND:Regulation of the receptor protein tyrosine phosphatase kappa (RPTP-κ) in the skin is essential, while transforming growth factor beta (TGF-β) appears to be an upstream factor of its regulation. As Notch signaling pathway is similar to TGF-βsignaling pathway, whether Notch participates in the regulation of RPTP-κtranscription by TGF-βsignaling? OBJECTIVE:To find out the role of Notch signal in regulation of RPTP-κtranscription by TGF-β. METHODS:Jagged-1 and gamma-secretase inhibitors (GSI) were use respectively to activate and inhibit Notch signal fol owed by addition of TGF-β. Simultaneously, control group was set. Real-time PCR was used to determine RPTP-k mRNA expression in human keratinocytes. RESULTS AND CONCLUSION:After adding TGF-βinto 40%confluent keratinocytes, the RPTP-κmRNA expression was higher at different time as compared with the control group. Fol owing Jagged-1 activated Notch signaling pathway, the mRNA expression of RPTP-κin the keratinocytes was higher when Jagged-1, TGF-βor their combination was added into the cel s as compared with the control group (P<0.05, P<0.01). Fol owing GSI inhibited Notch signaling pathway, the mRNA expression of RPTP-κin the keratinocytes was higher only when TGF-βwas added into the cel s as compared with the control group (P<0.01), and no significant difference was seen when GSI alone or combination of TGF-βand GSI was added into the cel s as compared with the control group (P>0.05). These findings indicate that the mRNA expression of RPTP-κin the keratinocytes was increased after TGF-βwas added, and fol owing activation or inhibition of Notch signaling, the mRNA expression of RPTP-κwas significantly increased or suppressed. Therefore, Notch signaling is very important and indispensable in the regulation of RPTP-κby TGF-β.