CAJ | 학술논문

目的探讨β-七叶皂甙钠(SA)对脊髓损伤(SCI)大鼠肢体功能及神经细胞凋亡的保护作用.方法采用改良Allen撞击方法建立SCI大鼠模型,随机将180只建模成功的SD大鼠按照数字随机表法分成对照组(等体积生理盐水腹腔注射)、SA组(5 mg/kg SA腹腔注射)及甲基强的松龙(MP)组(100 mg/kg MP腹腔注射),每组各60只.分别在治疗后8 h,1、4、7、14 d,利用斜板对双后肢瘫痪的恢复情况进行测定,并检测细胞凋亡的动态变化.结果随着观察时间的延长,各组大鼠后肢功能均有不同恢复,斜板角度逐渐增加.SA组和MP组治疗4 d后,大鼠后肢功能明显恢复,且呈时间依赖性(P<0.05).治疗后8 h、1 d,对照组与SA组大鼠脊髓神经细胞凋亡指数高于MP组(P<0.05);治疗后4、7、14 d,SA组与MP组大鼠脊髓神经细胞凋亡指数低于对照组(P<0.05).结论β-七叶皂甙钠对SCI大鼠肢体功能具有明显保护作用,其作用机理之一可能是通过减轻脊髓神经细胞凋亡来实现.
목적탐토β-칠협조대납(SA)대척수손상(SCI)대서지체공능급신경세포조망적보호작용.방법채용개량Allen당격방법건립SCI대서모형,수궤장180지건모성공적SD대서안조수자수궤표법분성대조조(등체적생리염수복강주사)、SA조(5 mg/kg SA복강주사)급갑기강적송룡(MP)조(100 mg/kg MP복강주사),매조각60지.분별재치료후8 h,1、4、7、14 d,이용사판대쌍후지탄탄적회복정황진행측정,병검측세포조망적동태변화.결과수착관찰시간적연장,각조대서후지공능균유불동회복,사판각도축점증가.SA조화MP조치료4 d후,대서후지공능명현회복,차정시간의뢰성(P<0.05).치료후8 h、1 d,대조조여SA조대서척수신경세포조망지수고우MP조(P<0.05);치료후4、7、14 d,SA조여MP조대서척수신경세포조망지수저우대조조(P<0.05).결론β-칠협조대납대SCI대서지체공능구유명현보호작용,기작용궤리지일가능시통과감경척수신경세포조망래실현.
Objective To investigate the protection effects of sodium aescinate (SA) on the limb's function and nerve cell apoptosis in the early spinal cord injuried (SCI) rats. Methods To establish the rat model of SCI with modified Allen method, a total of 180 SD rats were randomly divided into control group (normal saline intraperitoneal injection), SA group (5 mg/kg intraperitoneal injection of SA) and methylprednisolone (MP) group (100 mg/kg intraperitoneal injection of MP), with 60 rats in each group. At 8 h, 1, 4, 7, 14 d after treatment, slope degree were determined to evaluate the improvement of the function of paralyzed hindlimb, then the&nbsp