CAJ | 학술논문

目的研究心肌过表达肌浆网Ca2+-ATP酶(SERCA2a)基因对慢性心力衰竭犬神经内分泌系统的影响.方法超声心动图检测SERCA2a基因转导对慢性心力衰竭犬心脏收缩功能的影响,放射免疫法测定血管紧张素Ⅱ与心房利钠肽、内皮素-1、肿瘤坏死因子-α、白介素-6等神经内分泌因子.结果 SERCA2a基因转导后心力衰竭犬的心脏收缩功能得到改善(P<0.05),同时上述神经内分泌因子水平均减低(P<0.05).结论 SERCA2a过表达能改善心肌收缩功能,阻断神经内分泌系统的恶性循环,可能阻止或逆转心肌重塑.
목적연구심기과표체기장망Ca2+-ATP매(SERCA2a)기인대만성심력쇠갈견신경내분비계통적영향.방법초성심동도검측SERCA2a기인전도대만성심력쇠갈견심장수축공능적영향,방사면역법측정혈관긴장소Ⅱ여심방리납태、내피소-1、종류배사인자-α、백개소-6등신경내분비인자.결과 SERCA2a기인전도후심력쇠갈견적심장수축공능득도개선(P<0.05),동시상술신경내분비인자수평균감저(P<0.05).결론 SERCA2a과표체능개선심기수축공능,조단신경내분비계통적악성순배,가능조지혹역전심기중소.
@@@@Objective To explore the effect of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a) overexpression on neuroendocrine sys-tem in chronic heart failure beagles. Methods The cardiac contraction function of chronic heart failure beagles were assessed by echocar-diography before and after SERCA2a gene transduction. Radiation immune method was used to test the serum level of neuroendocrine fac-tors such as Angiotensin Ⅱ, atrial natriuretic peptide, endothelin-1 and TNF-α, IL-6. Results The cardiac contraction function of chronic heart failure beagles improved greatly (P<0.05), and the serum levels of neuroendocrine factors significantly reduced after SERCA2a over-expression (P<0.05). Conclusion The vicious circle of neuroendocrine system may be blocked partially by SERCA2a overexpression.