CAJ | 학술논문

目的观察参龙汤对脑缺血再灌注大鼠学习记忆能力及大鼠血浆神经肽(NPY)水平和海马NPY表达的影响.方法50只大鼠随机分为:假手术组,模型组,吡拉西坦组,参龙汤大、小剂量组,每组各10只.利用双侧颈总动脉结扎方法制备慢性脑缺血再灌注大鼠模型,造模后药物处理28 d,采用Morris水迷宫实验观察大鼠逃避潜伏期时间以及跨越平台次数,采用竞争酶联免疫分析法测定血浆NPY含量,采用免疫组化方法观察大鼠海马NPY表达的变化.结果与假手术组相比,模型组大鼠逃避潜伏期时间明显延长,跨越平台次数明显减少(P<0.01);与模型组相比,参龙汤大剂量组大鼠逃避潜伏期时间缩短,跨越平台次数增加(P<0.05).与假手术组相比,模型组大鼠血浆NPY含量明显升高,海马区NPY蛋白阳性细胞数明显升高(P<0.01);与模型组相比,治疗后参龙汤大剂量组和吡拉西坦组大鼠血浆NPY含量减低,海马区NPY蛋白阳性细胞数减低(P<0.05).结论240 g/L参龙汤能改善脑缺血再灌注大鼠学习记忆能力,其作用机理可能与降低大鼠血浆NPY含量、减少海马NPY蛋白表达有关.
목적관찰삼룡탕대뇌결혈재관주대서학습기억능력급대서혈장신경태(NPY)수평화해마NPY표체적영향.방법50지대서수궤분위:가수술조,모형조,필랍서탄조,삼룡탕대、소제량조,매조각10지.이용쌍측경총동맥결찰방법제비만성뇌결혈재관주대서모형,조모후약물처리28 d,채용Morris수미궁실험관찰대서도피잠복기시간이급과월평태차수,채용경쟁매련면역분석법측정혈장NPY함량,채용면역조화방법관찰대서해마NPY표체적변화.결과여가수술조상비,모형조대서도피잠복기시간명현연장,과월평태차수명현감소(P<0.01);여모형조상비,삼룡탕대제량조대서도피잠복기시간축단,과월평태차수증가(P<0.05).여가수술조상비,모형조대서혈장NPY함량명현승고,해마구NPY단백양성세포수명현승고(P<0.01);여모형조상비,치료후삼룡탕대제량조화필랍서탄조대서혈장NPY함량감저,해마구NPY단백양성세포수감저(P<0.05).결론240 g/L삼룡탕능개선뇌결혈재관주대서학습기억능력,기작용궤리가능여강저대서혈장NPY함량、감소해마NPY단백표체유관.
@@@@Objective To investigate the effect of Shenlongtang decoction (SLT) on the learning and memory ability and serum level of neuropeptide Y (NPY) and expression of NPY in hippocampus of rats with cerebral ischemia/reperfusion. Methods 50 rats were randomly divided into 5 groups: control group with sham operation; model group with cerebral ischemia/reperfusion; pisacetam group as positive con-trol; and SLT groups (low and high dosages). The rats' model was established with two-vessel occlusion. After modeling, the rats were ad-ministrating with SLT or pisacetam for 28 d. Learning and memory ability was tested with Morris water maze, the level of NPY was detect-ed with radioimmunoassay, and the number of positive cells of NPY in hippocampus was detected. Results Compared with the control group, the latency time increased significantly and frequence of searching submerged platform decreased significantly in the model group (P<0.01); and compared with the model group, the latency time decreased significantly and frequence of searching submerged platform in-creased significantly in SLT high dosage group (P<0.05). Compared with the control group, the serum levels of NPY increased significantly in the model group, and the number of positive cell of NPY in hippocampus area significantly increased (P<0.01); and compared with the model group, the serum levels of NPY decreased significantly, and the number of positive cell of NPY in hippocampus area significantly de-creased in the SLT high dosage group and pisacetam group (P<0.05). Conclusion SLT could improve the learning and memory function. The mechanism may be related to regulate the serum level and expression of NPY in hippocampus area of rats with cerebral ischemia/reper-fusion.