CAJ | 학술논문

目的探讨粒细胞集落刺激因子(G-CSF)结合行为训练对缺血性脑损伤大鼠的神经保护作用及对远期学习记忆能力的影响.方法2月龄清洁级 Wistar 大鼠96只,分为假手术组(A 组)、模型组(B 组),G-CSF 干预组(C 组)及 G-CSF+行为训练组(D组).采用改良线栓法制作大鼠大脑中动脉闭塞(MCAO)2 h 再灌注24 h 模型(A 组不插入线栓).各组分别于造模成功后1周、2周、4周、8周随机选取6只,利用穿梭实验视频分析系统计算逃避潜伏总时间,HE 染色和免疫组织化学染色观察造模后大鼠海马区病理变化及神经生长因子(NGF)、G-CSF 水平.结果造模后,B 组 NGF、G-CSF 表达短暂升高,C、D 组表达总体上高于 B 组,尤以 D 组为著(P<0.05);逃避潜伏总时间各组均延长,总体上 B 组长于 C 组,C 组长于 D 组(P<0.05).随着时间的延长,这一趋势更加明显.结论结合行为训练较单一应用 G-CSF 神经保护作用更强,且显著提高远期学习记忆能力.
목적탐토립세포집락자격인자(G-CSF)결합행위훈련대결혈성뇌손상대서적신경보호작용급대원기학습기억능력적영향.방법2월령청길급 Wistar 대서96지,분위가수술조(A 조)、모형조(B 조),G-CSF 간예조(C 조)급 G-CSF+행위훈련조(D조).채용개량선전법제작대서대뇌중동맥폐새(MCAO)2 h 재관주24 h 모형(A 조불삽입선전).각조분별우조모성공후1주、2주、4주、8주수궤선취6지,이용천사실험시빈분석계통계산도피잠복총시간,HE 염색화면역조직화학염색관찰조모후대서해마구병리변화급신경생장인자(NGF)、G-CSF 수평.결과조모후,B 조 NGF、G-CSF 표체단잠승고,C、D 조표체총체상고우 B 조,우이 D 조위저(P<0.05);도피잠복총시간각조균연장,총체상 B 조장우 C 조,C 조장우 D 조(P<0.05).수착시간적연장,저일추세경가명현.결론결합행위훈련교단일응용 G-CSF 신경보호작용경강,차현저제고원기학습기억능력.
@@@@Objective To investigate the neuroprotective effect of granulocyte colony stimulating factor (G-CSF) combined with behav-ior training on the learning and memory abilities of cerebral ischemic injury in rats. Methods 96 Wistar rats, 2 months old, were divided as sham group (group A), model group (group B), G-CSF group (group C) and G-CSF+training group (group D). Modified middle cerebral ar-tery occlusion (MCAO) was used to establish a ischemia 2 h/reperfusion 24 h model to all the rats, except sham group. 6 rats were selected in each group 1, 2, 4 and 8 weeks after successfully modeled, respectively. The abilities of learning and memory were detected with the la-tency of shuttle test video analysis system. The pathology of the hippocampus and the expression of nerve growth factor (NGF) and G-CSF were observed with HE and immunohistochemistry staining, respectively. Results The expression of NGF and G-CSF increased in group B transiently, and increased more and longer in the group C and D, especially in group D, as the time passed (P<0.05). The latency of shuttle test increased in group B, C and D, and gently decreased as the time passed, and the decrease more to less were group D, C and B (P<0.05). Conclusion The neuroprotective effects of G-CSF combined with behavior training are strengthened and long term beneficial than G-CSF alone.