CAJ | 학술논문

目的应用生物活性材料支架诱导神经再生修复成年大鼠创伤性脑损伤.方法52只成年雄性Wistar大鼠分为假手术组、单纯损伤组、单纯壳聚糖组和生物活性材料支架组,分别于术后3 d、7 d、2周、4周、2个月和3个月通过组织化学、免疫组织化学和神经示踪方法检测神经再生和损伤区新生神经元的来源.结果应用生物活性材料支架后,海马齿状回(DG)和室管膜下区(SVZ)中BrdU+/Nestin+和BrdU+/Dcx+细胞较单纯壳聚糖组和单纯损伤组明显增加.损伤区内 BrdU+/Nestin+、BrdU+/Dcx+及BrdU+/MAP2+神经细胞较单纯壳聚糖组和单纯损伤组明显增加.DiI标记SVZ细胞后,损伤区中见DiI+/NeuN+神经元.结论生物活性材料支架激活DG和SVZ的神经干细胞,SVZ中的神经干细胞迁移到损伤区,并分化为成熟神经元.
목적응용생물활성재료지가유도신경재생수복성년대서창상성뇌손상.방법52지성년웅성Wistar대서분위가수술조、단순손상조、단순각취당조화생물활성재료지가조,분별우술후3 d、7 d、2주、4주、2개월화3개월통과조직화학、면역조직화학화신경시종방법검측신경재생화손상구신생신경원적래원.결과응용생물활성재료지가후,해마치상회(DG)화실관막하구(SVZ)중BrdU+/Nestin+화BrdU+/Dcx+세포교단순각취당조화단순손상조명현증가.손상구내 BrdU+/Nestin+、BrdU+/Dcx+급BrdU+/MAP2+신경세포교단순각취당조화단순손상조명현증가.DiI표기SVZ세포후,손상구중견DiI+/NeuN+신경원.결론생물활성재료지가격활DG화SVZ적신경간세포,SVZ중적신경간세포천이도손상구,병분화위성숙신경원.
@@@@Objective To repair the brain of adult rats with traumatic brain injury by inducing neural regeneration with biological scaf-folds. Methods 52 adult male Wistar rats were divided into control group, lesion group, blank chitosan carriers group and bioactive material scaffolds group. The neural regeneration and the origin of the newborn neurons in the injury area were detected through histochemistry, im-mumochemistry and neural tracing on the 3rd day, 7th day, 2nd week, 4th week, 2nd month and 3rd month after operation. Results After ap-plication of bioactive material scaffolds, the BrdU+/Nestin+ and BrdU+/Dcx+ cells in gyrus dentatus (DG) of hippocampus and subventricular zone (SVZ) were significantly more than those of the other groups. The BrdU+/Nestin+, BrdU+/Dcx+ and BrdU+/MAP2+ neural cells in injury area of the bioactive material scaffolds group were significantly more than those of the other groups. After application of DiI to label the SVZ cells, DiI+/NeuN+ neurons were observed in the injury areas. Conclusion Bioactive material scaffolds can activate the neural stem cells in SVZ and DG. The neural stem cells in SVZ can migrate to the injury area and then differentiate into mature neurons.