CAJ | 학술논문

目的观察电针阿是穴对骨骼肌损伤大鼠腓肠肌增殖细胞核抗原(PCNA)、结蛋白(Desmin)及碱性成纤维细胞生长因子(bFGF)表达的影响,探讨电针阿是穴对骨骼肌损伤后肌卫星细胞增殖分化的可能机制.方法将78只雄性Sprague-Dawley大鼠随机分为模型组6只,空白组、电针组、自然修复组各24只,每组再分为1 d、4 d、7 d、14 d 4个亚组,各6只.模型组、自然修复组与电针组采用腓肠肌钝挫伤结合离心运动的方法造模,模型建立后,模型组取腓肠肌行HE染色,其余各组按时间点相应处理并取腓肠肌行PCNA、Desmin和bFGF免疫组化染色.结果自然修复组各时间点PCNA表达均明显高于空白组(P<0.01);Desmin在损伤后1 d、14 d低于空白组(P<0.05),损伤后7 d高于空白组(P>0.05);bFGF在损伤后7 d高于空白组(P<0.01).电针组PCNA、bFGF 与 Desmin 的表达在损伤后1 d、4 d 均高于自然修复组(P<0.05).结论电针阿是穴能促进肌卫星细胞增殖,加快其成肌分化,缩短损伤修复进程,其机制可能与上调及提前bFGF的表达有关.
목적관찰전침아시혈대골격기손상대서비장기증식세포핵항원(PCNA)、결단백(Desmin)급감성성섬유세포생장인자(bFGF)표체적영향,탐토전침아시혈대골격기손상후기위성세포증식분화적가능궤제.방법장78지웅성Sprague-Dawley대서수궤분위모형조6지,공백조、전침조、자연수복조각24지,매조재분위1 d、4 d、7 d、14 d 4개아조,각6지.모형조、자연수복조여전침조채용비장기둔좌상결합리심운동적방법조모,모형건립후,모형조취비장기행HE염색,기여각조안시간점상응처리병취비장기행PCNA、Desmin화bFGF면역조화염색.결과자연수복조각시간점PCNA표체균명현고우공백조(P<0.01);Desmin재손상후1 d、14 d저우공백조(P<0.05),손상후7 d고우공백조(P>0.05);bFGF재손상후7 d고우공백조(P<0.01).전침조PCNA、bFGF 여 Desmin 적표체재손상후1 d、4 d 균고우자연수복조(P<0.05).결론전침아시혈능촉진기위성세포증식,가쾌기성기분화,축단손상수복진정,기궤제가능여상조급제전bFGF적표체유관.
@@@@Objective To observe the effects of electroacupuncture at Ouch Point on proliferation cell nuclear antigen (PCNA), Desmin and basic fibroblast growth factor (bFGF) expression in gastrocnemius muscle, and explore the possible mechanisms of the effect. Methods 78 male Sprague-Dawley rats were randomly divided into model group (n=6), blank group (n=24), self-repair group (n=24) and electroacu-puncture group (n=24). Each of the later 3 groups was divided into 1 d, 4 d, 7 d and 14 d subgroups, 6 rats in each subgroup. The model group, self-repair group and electroacupuncture group were modeled by gastrocnemius muscle crush injury combined with eccentric exer-cise-induced injury. After that, the model group given HE stain, and the other groups were given correspondent approach according to time point and immunohistochemical technology was used to test the expression of PCNA, Desmin and bFGF. Results PCNA expression was higher in the self-repair group than in the blank group at each time point (P<0.01). Desmin expression was lower in the self-repair group than in the blank group 1 d and 14 d after injury (P<0.05), but higher 7 d after injury (P>0.05). bFGF expression was higher in the self-re-pair group than in the blank group 7 d after injury (P<0.01). PCNA, bFGF and Desmin expression were higher in the electroacupuncture group than in the self-repair group 1 d and 4 d after injury (P<0.05). Conclusion Electroacupuncture at Ouch Point can promote the prolifer-ation of muscle satellite cells, accelerate the myogenic differentiation and shorten the repair process, which may be related to the increased and advanced expression of bFGF.