CAJ | 학술논문

目的研究c-Jun氨基末端激酶(JNK)信号通路抑制对白细胞介素-1(IL-1)诱导的大鼠椎间盘纤维环细胞基因表达的影响,为阻止椎间盘退变提供新的治疗靶点.方法分离培养大鼠尾椎纤维环细胞,IL-1刺激纤维环细胞后,Western blot检测JNK信号通路的激活;传代的纤维环细胞培养48 h后,以IL-1(10 ng/ml)加或不加JNK通路抑制剂SP600125刺激24 h,Real-Time PCR检测JNK通路抑制对IL-1诱导的大鼠纤维环细胞基因表达的影响.结果 JNK通路抑制可显著抑制IL-1引起的诱导性一氧化氮合酶(iNOS)、白细胞介素-6(IL-6)、环氧合酶-2(Cox-2)和基质金属蛋白酶(MMP)-3、MMP-13表达上调,同时,可显著逆转IL-1引起的Ⅰ型胶原和胰岛素样生长因子-1(IGF-1)基因表达下调.结论 JNK通路抑制可作为阻断IL-1诱导的椎间盘退变的治疗靶点.
목적연구c-Jun안기말단격매(JNK)신호통로억제대백세포개소-1(IL-1)유도적대서추간반섬유배세포기인표체적영향,위조지추간반퇴변제공신적치료파점.방법분리배양대서미추섬유배세포,IL-1자격섬유배세포후,Western blot검측JNK신호통로적격활;전대적섬유배세포배양48 h후,이IL-1(10 ng/ml)가혹불가JNK통로억제제SP600125자격24 h,Real-Time PCR검측JNK통로억제대IL-1유도적대서섬유배세포기인표체적영향.결과 JNK통로억제가현저억제IL-1인기적유도성일양화담합매(iNOS)、백세포개소-6(IL-6)、배양합매-2(Cox-2)화기질금속단백매(MMP)-3、MMP-13표체상조,동시,가현저역전IL-1인기적Ⅰ형효원화이도소양생장인자-1(IGF-1)기인표체하조.결론 JNK통로억제가작위조단IL-1유도적추간반퇴변적치료파점.
@@@@Objective To determine the role of c-Jun N-terminal kinase (JNK) signaling in annulus fibrosus cells response to interleukin (IL)-1. Methods Rat annulus fibrosus cells cultured in monolayer were exposed to IL-1, with or without JNK inhibition by SP600125. RNA was isolated for Real-Time polymerase chain reaction (PCR) after 24 h stimulation. Results IL-1 upregulation of mRNA for inducible nitric oxide synthase (iNOS), IL-6, cyclooxygenase (Cox)-2, matrix metalloproteinase (MMP)-3, MMP-13 were blunted by JNK inhibition, while downregulation of collagenⅠand insulin-like growth factor (IGF)-1 were also reversed by JNK inhibition. Conclusion JNK signaling inhibi-tion can be a therapeutic target for blocking intervertebral disc degeneration induced by IL-1.