CAJ | 학술논문

目的探讨男性不育患者的精子DNA损伤率及其与精子活动率、活力、精子密度及快速向前运动的精子的相关性.方法精液标本来自正常对照者及不育患者,以WHO精液分析标准行精液分析,根据结果分为正常对照组、弱精子症组、少精子症组、少弱精子症组.用染色质扩散实验(SCD)检测精子DNA损伤率.结果正常对照组与不育组(包括弱精子症组、少精子症组和少弱精子症组)间的DNA损伤率有明显的差异(P均<0.05),不育组间DNA损伤率也存在差异(P<0.01).DNA损伤率与精子活动率、活力、快速向前运动的精子存在明显的负相关性(r=-0.484,r=-0.543,r=-0.475,P<0.01),与精子密度之间无相关性(P>0.05).结论随着男性精液质量的下降,精子DNA损伤率明显升高.精子DNA损伤可能是引起男性不育的重要原因之一,精子DNA损伤率的检测对评估男性生育能力和辅助生殖的结局有重要的临床意义.
목적탐토남성불육환자적정자DNA손상솔급기여정자활동솔、활력、정자밀도급쾌속향전운동적정자적상관성.방법정액표본래자정상대조자급불육환자,이WHO정액분석표준행정액분석,근거결과분위정상대조조、약정자증조、소정자증조、소약정자증조.용염색질확산실험(SCD)검측정자DNA손상솔.결과정상대조조여불육조(포괄약정자증조、소정자증조화소약정자증조)간적DNA손상솔유명현적차이(P균<0.05),불육조간DNA손상솔야존재차이(P<0.01).DNA손상솔여정자활동솔、활력、쾌속향전운동적정자존재명현적부상관성(r=-0.484,r=-0.543,r=-0.475,P<0.01),여정자밀도지간무상관성(P>0.05).결론수착남성정액질량적하강,정자DNA손상솔명현승고.정자DNA손상가능시인기남성불육적중요원인지일,정자DNA손상솔적검측대평고남성생육능력화보조생식적결국유중요적림상의의.
Objective To investigate the DNA damage frequency in subfertile patients, and explore the correlation between sperm DNA damage frequency and semen parameters including sperm viability, motility, sperm concentration and rapid forward progressive motility. Methods Semen samples were obtained from healthy normal and subfertile men. All sperm samples were divided into the normal group and the abnormal groups including asthenozoospermia group, oligozoospermia group and oligoasthenozoospermia group according to World Health Organization guidelines. Sperm DNA damage frequency was evaluated by the sperm chromatin dispersion (SCD) test. Results Significant differences were found in sperm DNA damage frequency between the control group and the subfertile groups ( P<0.01, P<0.05, P<0.01), and among in the subfertile groups (P<0.01). Significant correlations were determined between sperm DNA damage frequency and sperm viability, motility, rapid forward progressive motility (r=-0.484, r=-0.543, r=-0.475, P<0.01), but no correlations between sperm DNA damage frequency and sperm concentration(r=-0.155, P>0.05). Conclusion The DNA damage frequency increased significantly in parallel with the decrease in sperm quality. Sperm DNA damage might be an important reason for ocurrence of male infertility. The test of sperm DNA damage frequency was helpful to evaluate male fertility ability and the outcome of assisted reproductive technology.