CAJ | 학술논문

目的探索用化学发光方法评价精液中过量存在的白细胞对精液质量的影响.方法将男性不育患者分为精液参数正常组,弱精子症组和少弱精子症组,通过化学发光方法直接检测精浆活性氧(ROS)水平,以及检测白细胞特异探针fMLP激惹白细胞后精液白细胞的ROS水平.结果精浆ROS和白细胞所产生的ROS水平在各组之间无显著差别,但用白细胞ROS指数平衡了精浆ROS水平和精子密度的影响后,白细胞ROS指数在弱精子症组和少弱精子症组均显著高于精液参数正常组.结论精液中若存在相对于精子数量过量的白细胞,且白细胞经化合物(fMLP)刺激后产生ROS水平的能力过强,可能是导致精子活动力低下的重要原因.
목적탐색용화학발광방법평개정액중과량존재적백세포대정액질량적영향.방법장남성불육환자분위정액삼수정상조,약정자증조화소약정자증조,통과화학발광방법직접검측정장활성양(ROS)수평,이급검측백세포특이탐침fMLP격야백세포후정액백세포적ROS수평.결과정장ROS화백세포소산생적ROS수평재각조지간무현저차별,단용백세포ROS지수평형료정장ROS수평화정자밀도적영향후,백세포ROS지수재약정자증조화소약정자증조균현저고우정액삼수정상조.결론정액중약존재상대우정자수량과량적백세포,차백세포경화합물(fMLP)자격후산생ROS수평적능력과강,가능시도치정자활동력저하적중요원인.
Objective To evaluate the effects of leukocytes on semen quality. Methods Based on the parameters of semen analysis, the infertile males were divided into three groups such as the normal group, the athenospermia group and the oligozo-athenospermia group. Seminal ROS was detected by using chemiluminescence ,meanwhile the ability of WBC producing ROS in semen was determined by fMLP stimulating test. Results Among the three groups, no differences were found in the levels of ROS either in semen or produced by WBC. When the influence of seminal ROS and sperm concentration was normalized, the ROS index of WBC in both athenospermia group and oligozo-athenospermia groups were significantly higher than that in the normal group. Conclusion Relative to sperm concentration, high amount of seminal WBC and ROS level produced by WBC after fMLP stimulation might be the critical factors for decrease in the motility of sperm.