中国男科学杂志
中國男科學雜誌
중국남과학잡지
CHINESE JOURNAL OF ANDROLOGY
2013年
3期
34-37
,共4页
郑厚斌%江芳%王奇玲%张志兴%唐运革%邓顺美%刘晃%钟安%汤乐%唐立新**
鄭厚斌%江芳%王奇玲%張誌興%唐運革%鄧順美%劉晃%鐘安%湯樂%唐立新**
정후빈%강방%왕기령%장지흥%당운혁%산순미%류황%종안%탕악%당립신**
不育,男性%精索静脉曲张%脱氧鸟苷
不育,男性%精索靜脈麯張%脫氧鳥苷
불육,남성%정색정맥곡장%탈양조감
infertility,male%varicocele%deoxyguanosine
目的探讨8羟基脱氧鸟苷(8-hydroxy-2’-deoxyguanosine,8-OHdG)与男性不育精索静脉曲张(varicocele,VC)的相关性.方法选取在本院就诊男性不育VC的41例患者作为实验组,选取具有正常生育力男性33例作为对照组,按照WHO标准测定精液相关参数,利用8-OHdG特异性单克隆抗体试剂盒,应用双抗体夹心酶标免疫分析法测定患者精浆和静脉血中8-OHdG水平;分析8-OhdG与男性不育VC的相关性.结果对照组的精子浓度、前向运动率、存活率、正常形态率均高于实验组,差异具有统计学意义(P<0.05);对照组和实验组的血清8-OHdG水平分别为(44.03±29.23)ng/ml和(62.12±35.52)ng/ml;对照组和实验组的精浆8-OHdG水平分别为(18.68±3.36)ng/ml和(21.20±3.99)ng/ml;实验组精浆和血清8- OHdG水平均高于对照组.精浆8-OHdG与精子存活率、前向运动率呈现负相关关系(r=0.77,P<0.01; r=0.81, P=0.04).血清的8-OHdG水平高于精浆.数据显示患者年龄与精浆8-OhdG水平呈负相关(B=-0.225,P<0.05),年轻患者精浆8-OhdG水平较高.结论男性不育VC患者存在过氧化损伤,过量活性氧产生可能是患者精子质量低下的原因之一.VC与8-OHdG之间存在相关性,8-OHdG可作为一项过氧化检测指标,为临床男性不育诊疗提供有效的参考依据.
目的探討8羥基脫氧鳥苷(8-hydroxy-2’-deoxyguanosine,8-OHdG)與男性不育精索靜脈麯張(varicocele,VC)的相關性.方法選取在本院就診男性不育VC的41例患者作為實驗組,選取具有正常生育力男性33例作為對照組,按照WHO標準測定精液相關參數,利用8-OHdG特異性單剋隆抗體試劑盒,應用雙抗體夾心酶標免疫分析法測定患者精漿和靜脈血中8-OHdG水平;分析8-OhdG與男性不育VC的相關性.結果對照組的精子濃度、前嚮運動率、存活率、正常形態率均高于實驗組,差異具有統計學意義(P<0.05);對照組和實驗組的血清8-OHdG水平分彆為(44.03±29.23)ng/ml和(62.12±35.52)ng/ml;對照組和實驗組的精漿8-OHdG水平分彆為(18.68±3.36)ng/ml和(21.20±3.99)ng/ml;實驗組精漿和血清8- OHdG水平均高于對照組.精漿8-OHdG與精子存活率、前嚮運動率呈現負相關關繫(r=0.77,P<0.01; r=0.81, P=0.04).血清的8-OHdG水平高于精漿.數據顯示患者年齡與精漿8-OhdG水平呈負相關(B=-0.225,P<0.05),年輕患者精漿8-OhdG水平較高.結論男性不育VC患者存在過氧化損傷,過量活性氧產生可能是患者精子質量低下的原因之一.VC與8-OHdG之間存在相關性,8-OHdG可作為一項過氧化檢測指標,為臨床男性不育診療提供有效的參攷依據.
목적탐토8간기탈양조감(8-hydroxy-2’-deoxyguanosine,8-OHdG)여남성불육정색정맥곡장(varicocele,VC)적상관성.방법선취재본원취진남성불육VC적41례환자작위실험조,선취구유정상생육력남성33례작위대조조,안조WHO표준측정정액상관삼수,이용8-OHdG특이성단극륭항체시제합,응용쌍항체협심매표면역분석법측정환자정장화정맥혈중8-OHdG수평;분석8-OhdG여남성불육VC적상관성.결과대조조적정자농도、전향운동솔、존활솔、정상형태솔균고우실험조,차이구유통계학의의(P<0.05);대조조화실험조적혈청8-OHdG수평분별위(44.03±29.23)ng/ml화(62.12±35.52)ng/ml;대조조화실험조적정장8-OHdG수평분별위(18.68±3.36)ng/ml화(21.20±3.99)ng/ml;실험조정장화혈청8- OHdG수평균고우대조조.정장8-OHdG여정자존활솔、전향운동솔정현부상관관계(r=0.77,P<0.01; r=0.81, P=0.04).혈청적8-OHdG수평고우정장.수거현시환자년령여정장8-OhdG수평정부상관(B=-0.225,P<0.05),년경환자정장8-OhdG수평교고.결론남성불육VC환자존재과양화손상,과량활성양산생가능시환자정자질량저하적원인지일.VC여8-OHdG지간존재상관성,8-OHdG가작위일항과양화검측지표,위림상남성불육진료제공유효적삼고의거.
Objective To study the relationship of 8-hydroxy-2 ’-deoxyguanosine(8-OHdG) and male infertility with varicoceles(VC). Methods Total of 41 male infertility with varicoceles(the experiment group)and 33 well-bearing man(the control group) were enrolled in the study. The levels of 8-OHdG in blood and seminal plasma were detected by EIA. The relationship between 8-OHdG and VC was further explored. Results Sperm concentration, forward motility rate, survival rate and normal morphology of the control group were significantly higher than those of the experiment group. Blood 8-OHdG levels were (44.03±29.23) ng/ml and (62.12±35.52) ng/ml respectively (P<0.05). seminal plasma 8-OHdG levels were (18.68±3.36)ng/ml, (21.20±3.99)ng/ml respectively (P<0.05). Blood 8-OHdG levels were higher than seminal plasma 8-OHdG levels. 8-OHdG levels were negatively related to activity rates and survival rates(r=0.77, P<0.01;r=0.81, P=0.04) . 8-OHdG levels in seminal plasma of young man were higher than that of elders. Conclusion Peroxidative damages might be one reason of poor sperm quality in VC patients. 8-OHdG might be an indication for peroxidize reaction.
