中国生物防治学报
中國生物防治學報
중국생물방치학보
CHINESE JOURNAL OF BIOLOGICAL CONTROL
2013年
2期
227-231
,共5页
蜡蚧轮枝菌%农药%孢子萌发%菌丝生长%产孢
蠟蚧輪枝菌%農藥%孢子萌髮%菌絲生長%產孢
사개륜지균%농약%포자맹발%균사생장%산포
Verticillium lecanii%pesticides%conidia germination%mycelial growth%sporulation
真菌杀虫剂在有害生物综合治理中常与一些化学农药搭配使用.为了明确目前常用的农药品种与蜡蚧轮枝菌的相容性,本研究测定了北京市售的10种农药制剂对蜡蚧轮枝菌 Verticillium lecanii 孢子萌发、菌丝生长和产孢量的影响.对孢子毒力测试结果表明,45%咪鲜胺乳油、25.5%异菌脲悬浮剂、40%嘧霉胺悬浮剂和10.8%精喹禾灵乳油的毒力最强,LC50为2.27~5.58μg·mL?1;50%丙环唑可湿性粉剂、20%中生菌素可湿性粉剂、2.5%高渗吡虫啉可湿性粉剂和10.8%高效氟吡甲禾灵乳油的毒力居中,LC50为20.0~36.8μg·mL?1;72%2,4-滴丁酯乳油和48%氟乐灵乳油的毒力最弱,LC50分别为247.7μg·mL?1和376.62μg·mL?1.在田间推荐使用浓度下,供试农药制剂对蜡蚧轮枝菌孢子萌发、菌丝生长和产孢量均有显著抑制作用,其中2.5%高渗吡虫啉可湿性粉剂对孢子萌发的影响最小,抑制率为39.7%;20%中生菌素可湿性粉剂对菌丝生长和产孢量影响最小,抑制率分别为54.5%和34.8%.由此可知,蜡蚧轮枝菌与高渗吡虫啉和中生菌素具有一定的相容性,但相容值较低,田间应用需与之适当间隔,而与其余的8种农药则完全没有相容性.
真菌殺蟲劑在有害生物綜閤治理中常與一些化學農藥搭配使用.為瞭明確目前常用的農藥品種與蠟蚧輪枝菌的相容性,本研究測定瞭北京市售的10種農藥製劑對蠟蚧輪枝菌 Verticillium lecanii 孢子萌髮、菌絲生長和產孢量的影響.對孢子毒力測試結果錶明,45%咪鮮胺乳油、25.5%異菌脲懸浮劑、40%嘧黴胺懸浮劑和10.8%精喹禾靈乳油的毒力最彊,LC50為2.27~5.58μg·mL?1;50%丙環唑可濕性粉劑、20%中生菌素可濕性粉劑、2.5%高滲吡蟲啉可濕性粉劑和10.8%高效氟吡甲禾靈乳油的毒力居中,LC50為20.0~36.8μg·mL?1;72%2,4-滴丁酯乳油和48%氟樂靈乳油的毒力最弱,LC50分彆為247.7μg·mL?1和376.62μg·mL?1.在田間推薦使用濃度下,供試農藥製劑對蠟蚧輪枝菌孢子萌髮、菌絲生長和產孢量均有顯著抑製作用,其中2.5%高滲吡蟲啉可濕性粉劑對孢子萌髮的影響最小,抑製率為39.7%;20%中生菌素可濕性粉劑對菌絲生長和產孢量影響最小,抑製率分彆為54.5%和34.8%.由此可知,蠟蚧輪枝菌與高滲吡蟲啉和中生菌素具有一定的相容性,但相容值較低,田間應用需與之適噹間隔,而與其餘的8種農藥則完全沒有相容性.
진균살충제재유해생물종합치리중상여일사화학농약탑배사용.위료명학목전상용적농약품충여사개륜지균적상용성,본연구측정료북경시수적10충농약제제대사개륜지균 Verticillium lecanii 포자맹발、균사생장화산포량적영향.대포자독력측시결과표명,45%미선알유유、25.5%이균뇨현부제、40%밀매알현부제화10.8%정규화령유유적독력최강,LC50위2.27~5.58μg·mL?1;50%병배서가습성분제、20%중생균소가습성분제、2.5%고삼필충람가습성분제화10.8%고효불필갑화령유유적독력거중,LC50위20.0~36.8μg·mL?1;72%2,4-적정지유유화48%불악령유유적독력최약,LC50분별위247.7μg·mL?1화376.62μg·mL?1.재전간추천사용농도하,공시농약제제대사개륜지균포자맹발、균사생장화산포량균유현저억제작용,기중2.5%고삼필충람가습성분제대포자맹발적영향최소,억제솔위39.7%;20%중생균소가습성분제대균사생장화산포량영향최소,억제솔분별위54.5%화34.8%.유차가지,사개륜지균여고삼필충람화중생균소구유일정적상용성,단상용치교저,전간응용수여지괄당간격,이여기여적8충농약칙완전몰유상용성.
Fungal insecticides are often used with chemical pesticides in integrated pest management. Effects of ten pesticides on current Beijing market on conidial germination, mycelial growth and sporulation of Verticillium lecanii were tested in order to investigate the compatibility between the fungus and the pesticides. The results showed that prochloraz 45% EC, iprodione 25.5% SC, pyrimethanil 40% SC and quizalofop-p-ethyl 10.8% EC had highest toxicity to conidial germination of the fungus with LC50 ranging from 2.27-5.58 μg·mL?1. The LC50 of propiconazol 50% WP, zhongshengmycin 20% WP, imidacloprid 25% WP and haloxyfop-R-methyl 10.8% EC were middle, ranging from 20.0-36.8 μg·mL?1. Toxicity of 2,4-D butylate 72% EC and trifluralin 48% EC to conidial germination of the fungus were lowest with LC50 247.7 μg·mL?1 and 376.62 μg·mL?1 respectively. At the field recommended concentration, all pesticides had significant influence on conidial germination, mycelial growth and sporulation of the fungus. Imidacloprid 25% WP had the lowest effect on conidial germination, the inhibiton rate by which was 39.7%. Moreover zhongshengmycin 20% WP had the lowest effect on mycelial growth and sporulation with the inhibition rate 54.5% and 34.8% respectively. Consequently imidacloprid 25% WP and zhongshengmycin 
