CAJ | 학술논문

为了解生防哈茨木霉 Tr-92诱导黄瓜抗灰霉病的作用机制,采用生理生化法测定了哈茨木霉Tr-92诱导处理黄瓜根部后对叶部组织中的过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、β-1,3-葡聚糖酶(β-1,3-glucanase)以及几丁质酶(chitinase)活性的影响,通过 SDS-PAGE测定了诱导处理后不同时间叶部组织中过氧化物酶同工酶和病程相关蛋白的变化.结果表明,经哈茨木霉 Tr-92诱导处理后,黄瓜叶部组织中的过氧化物酶、多酚氧化酶、苯丙氨酸解氨酶、β-1,3-葡聚糖酶以及几丁质酶的活性均高于其它处理,哈茨木霉 Tr-92诱导处理对过氧化物酶同工酶的表达也有一定的影响,在诱导处理后的120 h 内,随着诱导时间的延长,黄瓜叶片中过氧化物酶同工酶主酶带逐渐加宽加深,并产生了一条新的条带,叶片中病程相关蛋白也出现部分蛋白条带颜色加深加宽的现象,并出现了两条新条带,这说明哈茨木霉 Tr-92诱导处理黄瓜根部后,能够影响黄瓜叶部相关防御酶系和部分病程相关蛋白的表达.由此表明,哈茨木霉 Tr-92可能通过改变这些物质的含量来提高黄瓜对灰霉病的抗性.
위료해생방합자목매 Tr-92유도황과항회매병적작용궤제,채용생리생화법측정료합자목매Tr-92유도처리황과근부후대협부조직중적과양화물매(POD)、다분양화매(PPO)、분병안산해안매(PAL)、β-1,3-포취당매(β-1,3-glucanase)이급궤정질매(chitinase)활성적영향,통과 SDS-PAGE측정료유도처리후불동시간협부조직중과양화물매동공매화병정상관단백적변화.결과표명,경합자목매 Tr-92유도처리후,황과협부조직중적과양화물매、다분양화매、분병안산해안매、β-1,3-포취당매이급궤정질매적활성균고우기타처리,합자목매 Tr-92유도처리대과양화물매동공매적표체야유일정적영향,재유도처리후적120 h 내,수착유도시간적연장,황과협편중과양화물매동공매주매대축점가관가심,병산생료일조신적조대,협편중병정상관단백야출현부분단백조대안색가심가관적현상,병출현료량조신조대,저설명합자목매 Tr-92유도처리황과근부후,능구영향황과협부상관방어매계화부분병정상관단백적표체.유차표명,합자목매 Tr-92가능통과개변저사물질적함량래제고황과대회매병적항성.
@@@@For elucidating the mechanism of induced systemic resistance of cucumber to grey mould by Trichoderma harzianum, we determinated the activity of peroxidase, polyphenol oxidase, L-phenylalaniammo-nialyase, β-1,3-glucanese and chitinase in cucumber leaves. We also monitored POD isozyme and pathogenesis- related proteins (PRS) in cucumber leaves by SDS-PAGE after treatment of cucumber roots by T. harzianum Tr-92. Results suggested that after inoculation with Tr-92, activities of POD, PPO, PAL,β-1,3-glucanase and chitinase in cucumber leaves were higher than that of control in 120h POD isozyme and PRS in cucumber leaves changed significantly. SDS-PAGE showed that a new band of POD isozyme appeared, and two new PRS bands appeared in extract of cucumber leaves after induced by Tr-92. It was suggested that change of these enzymes’ activities might be involved in the induced disease resistance of cucumber to grey mold by T. harzianum Tr-92.