CAJ | 학술논문

对前期筛选获得和在田间试验中防治水稻细菌性条斑病效果较好的解淀粉芽胞杆菌 Bacillus amyloliquefaciens Lx-11,通过 Plackett-Burman 试验设计及 Box-Behnken 设计的响应曲面法(response surface methodology, RSM)对影响生防菌株 Lx-11发酵培养基和培养条件进行了优化.培养基3个主要因子的质量浓度分别为玉米淀粉11.2 g·L?1、黄豆粉14.6 g·L?1和酵母膏0.62 g·L?1.培养条件优化试验得出:培养温度、通气量和接种量是影响发酵活菌数的主要因子,由所得响应曲面方程预测出这3个主要因子分别为30.84°C、59.68 mL/250 mL 和1.52%时,在初始 pH 为7.0,150 r·min?1培养48 h 的条件下,发酵活菌数和抑菌带宽的最大值分别为3.75×109 cfu·mL?1和8.2 mm.经摇瓶发酵试验和抑菌活性验证该理论预测值与实际值无显著差异.
대전기사선획득화재전간시험중방치수도세균성조반병효과교호적해정분아포간균 Bacillus amyloliquefaciens Lx-11,통과 Plackett-Burman 시험설계급 Box-Behnken 설계적향응곡면법(response surface methodology, RSM)대영향생방균주 Lx-11발효배양기화배양조건진행료우화.배양기3개주요인자적질량농도분별위옥미정분11.2 g·L?1、황두분14.6 g·L?1화효모고0.62 g·L?1.배양조건우화시험득출:배양온도、통기량화접충량시영향발효활균수적주요인자,유소득향응곡면방정예측출저3개주요인자분별위30.84°C、59.68 mL/250 mL 화1.52%시,재초시 pH 위7.0,150 r·min?1배양48 h 적조건하,발효활균수화억균대관적최대치분별위3.75×109 cfu·mL?1화8.2 mm.경요병발효시험화억균활성험증해이론예측치여실제치무현저차이.
@@@@The Plackett-Burman experiments and response surface methodology were adopted to optimize the liquid fermentation medium and conditions of the biocontrol agent of Bacillus amyloliquefaciens Lx-11. According to the evaluation by the response surface model established in this study, the optimum mass concentrations of the three components, corn starch, soyean powder and yeast extract, in fermentation medium were 11.2 g·L?1, 14.6 g·L?1 and 0.62 g·L?1, respectively. The fermentation condition determination tests indicated that temperature, filling volume of shaking flask and inoculum volume were the major factors affecting Lx-11 yield. The optimized fermentation conditions were 30.84 °C, inoculum volume 1.52% and the filling volume 59.68 mL in 250 mL shaking flask for 48 h at 150 r·min-1, and initial pH 7.0. Under the conditions spore production was up to 3.75×109 cfu·mL?1 and the width of antibacterial activity was 8.2 mm. Fermentation experiments with shake flasks verified that there was no statistical difference between real and forcasted yields.