中国生物防治学报
中國生物防治學報
중국생물방치학보
CHINESE JOURNAL OF BIOLOGICAL CONTROL
2013年
2期
270-276
,共7页
曹琦琦*%周登博*%郑丽%杨媚%周而勋**
曹琦琦*%週登博*%鄭麗%楊媚%週而勛**
조기기*%주등박*%정려%양미%주이훈**
水稻纹枯病%立枯丝核菌%拮抗菌%菌种鉴定%发酵条件
水稻紋枯病%立枯絲覈菌%拮抗菌%菌種鑒定%髮酵條件
수도문고병%립고사핵균%길항균%균충감정%발효조건
rice sheath blight%Rhizoctonia solani%antagonistic microbe%species identification%fermentation conditions
从不同土壤、植物和水稻纹枯病菌 Rhizoctonia solani 菌核样品上分离到细菌菌株325株和放线菌菌株86株.通过琼脂平板对峙法及发酵滤液介质筛选法,获得了对水稻纹枯病菌具有较强拮抗活性的细菌和放线菌菌株各1株,它们对水稻纹枯病菌菌丝生长的抑制率分别为75.56%和84.07%.采用形态学和生理生化学以及分子生物学方法,将细菌菌株 NB12鉴定为枯草芽胞杆菌 Bacillus subtilis、放线菌菌株 NA1鉴定为 Streptomyces triostinicus.对它们产生抑菌物质的发酵条件进行了探索,明确了菌株 NB12的最佳发酵条件为:初始 pH 7.0的 LB 或 BPY 培养液、装液量40 mL/250 mL、培养温度30℃、摇床转速180 r·min?1、培养时间48 h;菌株 NA1的最佳发酵条件为:初始 pH 6.0~9.0的大豆粉培养液或大豆粉–玉米粉培养液、装液量130 mL/250 mL、培养温度35℃、摇床转速140 r·min?1、培养时间≥72 h.
從不同土壤、植物和水稻紋枯病菌 Rhizoctonia solani 菌覈樣品上分離到細菌菌株325株和放線菌菌株86株.通過瓊脂平闆對峙法及髮酵濾液介質篩選法,穫得瞭對水稻紋枯病菌具有較彊拮抗活性的細菌和放線菌菌株各1株,它們對水稻紋枯病菌菌絲生長的抑製率分彆為75.56%和84.07%.採用形態學和生理生化學以及分子生物學方法,將細菌菌株 NB12鑒定為枯草芽胞桿菌 Bacillus subtilis、放線菌菌株 NA1鑒定為 Streptomyces triostinicus.對它們產生抑菌物質的髮酵條件進行瞭探索,明確瞭菌株 NB12的最佳髮酵條件為:初始 pH 7.0的 LB 或 BPY 培養液、裝液量40 mL/250 mL、培養溫度30℃、搖床轉速180 r·min?1、培養時間48 h;菌株 NA1的最佳髮酵條件為:初始 pH 6.0~9.0的大豆粉培養液或大豆粉–玉米粉培養液、裝液量130 mL/250 mL、培養溫度35℃、搖床轉速140 r·min?1、培養時間≥72 h.
종불동토양、식물화수도문고병균 Rhizoctonia solani 균핵양품상분리도세균균주325주화방선균균주86주.통과경지평판대치법급발효려액개질사선법,획득료대수도문고병균구유교강길항활성적세균화방선균균주각1주,타문대수도문고병균균사생장적억제솔분별위75.56%화84.07%.채용형태학화생리생화학이급분자생물학방법,장세균균주 NB12감정위고초아포간균 Bacillus subtilis、방선균균주 NA1감정위 Streptomyces triostinicus.대타문산생억균물질적발효조건진행료탐색,명학료균주 NB12적최가발효조건위:초시 pH 7.0적 LB 혹 BPY 배양액、장액량40 mL/250 mL、배양온도30℃、요상전속180 r·min?1、배양시간48 h;균주 NA1적최가발효조건위:초시 pH 6.0~9.0적대두분배양액혹대두분–옥미분배양액、장액량130 mL/250 mL、배양온도35℃、요상전속140 r·min?1、배양시간≥72 h.
@@@@Three hundred and twenty five bacterial strains and eighty six actinomycete strains were isolated from the samples taken from different soils, plants and Rhizoctonia solani sclerotia. One bacterial and one actinomycete strains with strong antagonistic activities to R. solani were selected by using both agar plate dual culture and fermentation filtrate-amended medium screening methods. Their inhibition rates to mycelial growth of R. solani were 75.56% and 84.07%, respectively, on the two medial plates. Based on morphological, physiological, biochemical and molecular characteristics, NB12 was identified as Bacillus subtilis, and NA1 as Streptomyces triostinicus. The cultivation conditions for antifungal substance production by NB12 were: LB or BPY medium at initial pH 7.0, culture volume at 40 mL/250 mL flask, incubation temperature at 30 ?C, and shaking rate at 180 r·min?1 for 48 h; whereas those for NA1 were: soybean or soybean–corn powder liquid medium at initial pH 6.0—9.0, culture volume at 130 mL/250 mL flask, incubation temperature at 35 ?C, and shaking rate at 140 r·min?1 for 72 h or above.
