中国血液流变学杂志
中國血液流變學雜誌
중국혈액류변학잡지
CHINESE JOURNAL OF HEMORHEOLOGY
2013年
1期
24-26
,共3页
内皮细胞蛋白C受体%IL-1β%曲格列酮
內皮細胞蛋白C受體%IL-1β%麯格列酮
내피세포단백C수체%IL-1β%곡격렬동
endothelial cell protein C receptor(EPCR)%IL-1β%troglitazone
目的探讨内皮细胞蛋白C受体(EPCR)在人脐静脉内皮细胞(ECV304)上的表达及炎症因子白介素-1β(IL-1β),PPARγ激动剂曲格列酮对其影响.方法采用流式细胞仪检测ECV304中EPCR的表达;实时定量PCR技术(Real-Time PCR)检测IL-1β作用后ECV304细胞EPCR mRNA表达;曲格列酮和IL-1β共同作用于ECV304细胞,实时定量PCR技术检测EPCR mRNA表达.结果 EPCR在ECV304中有很高表达,IL-1β下调ECV304细胞EPCR的表达,而曲格列酮可以明显改善IL-1β对EPCR的抑制.结论 ECV304中EPCR的表达可受到炎症因子IL-1β的影响,曲格列酮对上述影响有一定的拮抗作用,对ECV304损伤有保护作用.
目的探討內皮細胞蛋白C受體(EPCR)在人臍靜脈內皮細胞(ECV304)上的錶達及炎癥因子白介素-1β(IL-1β),PPARγ激動劑麯格列酮對其影響.方法採用流式細胞儀檢測ECV304中EPCR的錶達;實時定量PCR技術(Real-Time PCR)檢測IL-1β作用後ECV304細胞EPCR mRNA錶達;麯格列酮和IL-1β共同作用于ECV304細胞,實時定量PCR技術檢測EPCR mRNA錶達.結果 EPCR在ECV304中有很高錶達,IL-1β下調ECV304細胞EPCR的錶達,而麯格列酮可以明顯改善IL-1β對EPCR的抑製.結論 ECV304中EPCR的錶達可受到炎癥因子IL-1β的影響,麯格列酮對上述影響有一定的拮抗作用,對ECV304損傷有保護作用.
목적탐토내피세포단백C수체(EPCR)재인제정맥내피세포(ECV304)상적표체급염증인자백개소-1β(IL-1β),PPARγ격동제곡격렬동대기영향.방법채용류식세포의검측ECV304중EPCR적표체;실시정량PCR기술(Real-Time PCR)검측IL-1β작용후ECV304세포EPCR mRNA표체;곡격렬동화IL-1β공동작용우ECV304세포,실시정량PCR기술검측EPCR mRNA표체.결과 EPCR재ECV304중유흔고표체,IL-1β하조ECV304세포EPCR적표체,이곡격렬동가이명현개선IL-1β대EPCR적억제.결론 ECV304중EPCR적표체가수도염증인자IL-1β적영향,곡격렬동대상술영향유일정적길항작용,대ECV304손상유보호작용.
Objective To investigate the effect of IL-1β on Endothelial cell protein C receptor(EPCR) expression in umbilical vein endothelial cells.Methods EPCR expression was assessed by flow cytometry in umbilical vein endothelial cells.The effects of IL-1β and troiglitazone were measured by Real-Time PCR. Results Umbilical vein endothelial cells have a high expression of EPCR.IL-1β reduce EPCR expression.And troglitazone could significantly improve the inhibition.Conclusion We observed EPCR expression in umbilical vein endothelial cells in vitro.Some factors such as IL-1β can impact on EPCR.However,troglitazone had protective effects of EPCR on injured cells.
