CAJ | 학술논문

目的运用液体蛋白芯片飞行时间质谱(MALDI-TOF-MS)技术分析19片段角蛋白(CYFRA21-1)阴性的肺鳞癌血清蛋白质表达谱,筛选具有诊断意义的血清标志物,用于CYFRA21-1阴性的肺鳞癌的早期诊断.方法采集60例CYFRA21-1阴性肺鳞癌患者和60名正常对照者血清标本,随机分为模型构建组和模型验证组;运用Clinprot磁珠纯化、MALDI-TOF-MS技术及ClinProTools软件检测分析模型构建组血清蛋白表达谱,筛选差异蛋白并建立诊断模型,再用模型验证组标本进行验证.结果模型构建组CYFRA21-1阴性的肺鳞癌患者与正常对照者的血清蛋白质图谱比较,发现差异蛋白23个,筛选出质荷比为3883.83(m/z)、4640.68(m/z)、6629.27(m/z)、7763.29(m/z)、9284.66(m/z)的蛋白建立诊断模型,模型敏感性为90.0%,特异性为96.7%,其中差异蛋白6629.27(m/z)为肿瘤高表达,差异蛋白3883.83(m/z)、4640.68(m/z)、7763.29(m/z)、9284.66(m/z)为肿瘤低表达.结论运用MALDI-TOF-MS技术可筛选出CYFRA21-1阴性的肺鳞癌血清中的特异性标志物,为肺鳞癌的早期诊断提供了新的检测方法.
목적운용액체단백심편비행시간질보(MALDI-TOF-MS)기술분석19편단각단백(CYFRA21-1)음성적폐린암혈청단백질표체보,사선구유진단의의적혈청표지물,용우CYFRA21-1음성적폐린암적조기진단.방법채집60례CYFRA21-1음성폐린암환자화60명정상대조자혈청표본,수궤분위모형구건조화모형험증조;운용Clinprot자주순화、MALDI-TOF-MS기술급ClinProTools연건검측분석모형구건조혈청단백표체보,사선차이단백병건립진단모형,재용모형험증조표본진행험증.결과모형구건조CYFRA21-1음성적폐린암환자여정상대조자적혈청단백질도보비교,발현차이단백23개,사선출질하비위3883.83(m/z)、4640.68(m/z)、6629.27(m/z)、7763.29(m/z)、9284.66(m/z)적단백건립진단모형,모형민감성위90.0%,특이성위96.7%,기중차이단백6629.27(m/z)위종류고표체,차이단백3883.83(m/z)、4640.68(m/z)、7763.29(m/z)、9284.66(m/z)위종류저표체.결론운용MALDI-TOF-MS기술가사선출CYFRA21-1음성적폐린암혈청중적특이성표지물,위폐린암적조기진단제공료신적검측방법.
Objective To establish proteomic profiling using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) and to seek biomarkers of CYFRA21-1 negative human lung squamous for early diagnosis.Methods The serum samples of 60 CYFRA21-1 negative human lung squamous carcinoma patients and 60 normal control subjects were randomly divided into two groups to build up the group of establishing model and the group of verifying model.The serum samples of establishing model were analyzed by the technique of MALDI-TOF-MS and ClinProTools software to screen the differential proteins and establish the diagnostic model.The differential proteins and the model were verified by the groups of verifying model. The interested proteins were identified.Results The serum protein spectrums in the group of establishing model including CYFRA21-1 negative human lung squamous carcinoma patients and normal control subjects were compared and got 23 differential proteins.The model was established and the sensitivity and specificity was 90.0%and 96.7% respectively.The up-regulated proteins were 6629.27(m/z);The down-regulated protein was 3883.83(m/z),4640.68(m/z),7763.29(m/z) and 9284.66(m/z).Conclusion The biological markers in the earlier period of CYFRA21-1 negative human lung squamous carcinoma can be detected by the technology of MALDI-TOF-MS and the software of ClinProTools.It could be a new,convenient analyzing method in the early diagnosis and therapy of human lung squamous carcinoma.