CAJ | 학술논문

目的该研究在糖耐量正常人群中分别采用化学发光法及放射免疫法测定空腹及精氨酸(Arg)刺激后各点血清胰岛素(Ins)、C肽(Cp)水平的变化,并建立精氨酸指数(AIR-Arg)的正常参考值,为临床的应用提供依据.方法70名居住在上海地区的汉族人(男22名/女48名,年龄22~55岁).分别采用化学发光法及放射免疫法测定Arg刺激后0、2、4、6min血清Ins、Cp水平,以Arg刺激后Ins、Cp增值(AIR)反映β细胞快速分泌功能,将NGT者AIR-Ins、AIR-Cp的下1/4位点作为正常参考值.结果①Arg刺激后正常糖耐量者的Ins、CP水平迅速升高,在2~4min内分泌达到高峰,随即迅速下降.血糖则表现出轻度升高,与前两者上升趋势不平行.②分别用化学发光法及放射免疫法测定的正常人AIR参考值:AIR-Ins(化学发光法)≥24.07mU/L,AIR-Cp(化学发光法)≥2.1ng/mL及AIR-Ins(放射免疫法)≥30.94mU/L,AIR-Cp (放射免疫法)≥1.9ng/mL,呈非正态分布.③用两种方法测定的AIR-Ins、AIR-Cp呈显著正相关.结论 AIR-Arg可作为胰岛快速分泌相的指标,反映胰岛β细胞功能的异常与否.测定的正常人AIR-Arg结果可作为该实验室的正常参考值,为临床评估提供依据.
목적해연구재당내량정상인군중분별채용화학발광법급방사면역법측정공복급정안산(Arg)자격후각점혈청이도소(Ins)、C태(Cp)수평적변화,병건립정안산지수(AIR-Arg)적정상삼고치,위림상적응용제공의거.방법70명거주재상해지구적한족인(남22명/녀48명,년령22~55세).분별채용화학발광법급방사면역법측정Arg자격후0、2、4、6min혈청Ins、Cp수평,이Arg자격후Ins、Cp증치(AIR)반영β세포쾌속분비공능,장NGT자AIR-Ins、AIR-Cp적하1/4위점작위정상삼고치.결과①Arg자격후정상당내량자적Ins、CP수평신속승고,재2~4min내분비체도고봉,수즉신속하강.혈당칙표현출경도승고,여전량자상승추세불평행.②분별용화학발광법급방사면역법측정적정상인AIR삼고치:AIR-Ins(화학발광법)≥24.07mU/L,AIR-Cp(화학발광법)≥2.1ng/mL급AIR-Ins(방사면역법)≥30.94mU/L,AIR-Cp (방사면역법)≥1.9ng/mL,정비정태분포.③용량충방법측정적AIR-Ins、AIR-Cp정현저정상관.결론 AIR-Arg가작위이도쾌속분비상적지표,반영이도β세포공능적이상여부.측정적정상인AIR-Arg결과가작위해실험실적정상삼고치,위림상평고제공의거.
Objective To perform arginine stimulation test in subjects with normal glucose tolerance and measure plasma insulin and C-peptide level with chemiluminescence method and radioimmunoassay,respectively to establish the normal reference range of Arginine index.Methods Arginine stimulation test were performed in 70 individuals(men/women:22/48,age:22~55) who participated a physical examination in our hospital. Serum true insulin and C-peptide were measured at fasting and 2,4,6 minutes after injection of arginine with chemoluminescence method and radioimmunoassay,respectively and arginine index was calculated.Results ①The reference range of arginine index(true insulin secretion and C-peptide secretion) was more than 24.07mU/L and 2.1ng/mL(chemiluminescence method),30.94mU/L and 1.9ng/mL(radioimmunoassay),respectively,the range was normal distribution.②After 2~4 minutes injection of arginine,true insulin and C-peptide secretion reached the peak and decreased immediately.The blood glucose levels raised slightly and was not paralleled to elevate true insulin and C-peptide.③The result of true insulin and C-peptide measured with chemoluminescence method and radioimmunoassay were significantly correlated.Conclusion The arginine stimulation test enable estimateβcell secretory capacity.Arginine index could be used as reliable parameters in evaluating the early phase insulin secretion.