中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2013年
14期
78-79
,共2页
膝骨关节炎%软骨退变%丝氨酸蛋白酶1%关节滑液
膝骨關節炎%軟骨退變%絲氨痠蛋白酶1%關節滑液
슬골관절염%연골퇴변%사안산단백매1%관절활액
Knee Osteoarthritis%Cartilage degeneration%High temperature requirement factor 1%Apoptosis synovial fluid
目的:探讨骨关节炎患者关节滑液中HtrA1的表达是否与软骨退变程度相关.方法:研究对象包括膝骨关节炎(KOA)患者40例,其中软骨退变Ⅱ、Ⅲ、Ⅳ级的病例数分别为15、11、14例,正常人28例,应用ELISA测定丝氨酸蛋白酶1(HtrA1)在关节滑液中的表达程度.结果:KOAⅡ、Ⅲ、Ⅳ组中关节滑液的HtrA1因子表达均明显高于正常人,差异有统计学意义(P<0.01),而不同软骨退变程度患者的关节液HtrA1因子表达也不同.KOAⅡ、Ⅲ、Ⅳ组的组间HtrA1因子表达含量比较差异无统计学意义(P>0.05).正常对照组关节液HtrA1因子表达含量为67.89~208.33 pg/ml,以208.33 pg/ml作为阴性,KOA患者不同程度软骨退变(KOAⅡ、Ⅲ、Ⅳ组)各组检测的敏感度均超过85.7%.结论:HtrA1对早期软骨退变(KOAⅢ级)诊断的敏感度在90%以上,特别是诊断KOA患者KOAⅡ级软骨退变的敏感度最高,可达93%以上.说明HtrA1是诊断早期软骨退变(KOAⅡ级)的良好指标.
目的:探討骨關節炎患者關節滑液中HtrA1的錶達是否與軟骨退變程度相關.方法:研究對象包括膝骨關節炎(KOA)患者40例,其中軟骨退變Ⅱ、Ⅲ、Ⅳ級的病例數分彆為15、11、14例,正常人28例,應用ELISA測定絲氨痠蛋白酶1(HtrA1)在關節滑液中的錶達程度.結果:KOAⅡ、Ⅲ、Ⅳ組中關節滑液的HtrA1因子錶達均明顯高于正常人,差異有統計學意義(P<0.01),而不同軟骨退變程度患者的關節液HtrA1因子錶達也不同.KOAⅡ、Ⅲ、Ⅳ組的組間HtrA1因子錶達含量比較差異無統計學意義(P>0.05).正常對照組關節液HtrA1因子錶達含量為67.89~208.33 pg/ml,以208.33 pg/ml作為陰性,KOA患者不同程度軟骨退變(KOAⅡ、Ⅲ、Ⅳ組)各組檢測的敏感度均超過85.7%.結論:HtrA1對早期軟骨退變(KOAⅢ級)診斷的敏感度在90%以上,特彆是診斷KOA患者KOAⅡ級軟骨退變的敏感度最高,可達93%以上.說明HtrA1是診斷早期軟骨退變(KOAⅡ級)的良好指標.
목적:탐토골관절염환자관절활액중HtrA1적표체시부여연골퇴변정도상관.방법:연구대상포괄슬골관절염(KOA)환자40례,기중연골퇴변Ⅱ、Ⅲ、Ⅳ급적병례수분별위15、11、14례,정상인28례,응용ELISA측정사안산단백매1(HtrA1)재관절활액중적표체정도.결과:KOAⅡ、Ⅲ、Ⅳ조중관절활액적HtrA1인자표체균명현고우정상인,차이유통계학의의(P<0.01),이불동연골퇴변정도환자적관절액HtrA1인자표체야불동.KOAⅡ、Ⅲ、Ⅳ조적조간HtrA1인자표체함량비교차이무통계학의의(P>0.05).정상대조조관절액HtrA1인자표체함량위67.89~208.33 pg/ml,이208.33 pg/ml작위음성,KOA환자불동정도연골퇴변(KOAⅡ、Ⅲ、Ⅳ조)각조검측적민감도균초과85.7%.결론:HtrA1대조기연골퇴변(KOAⅢ급)진단적민감도재90%이상,특별시진단KOA환자KOAⅡ급연골퇴변적민감도최고,가체93%이상.설명HtrA1시진단조기연골퇴변(KOAⅡ급)적량호지표.
Objective:To explore the relationship of HtrA1 expression of synovial fluid in osteoarthritis and the degree of cartilage degeneration. Method:The object of study,including 40 cases of patients with knee osteoarthritis,including cartilage degeneration gradeⅡ,Ⅲ,gradeⅣnumber of cases were 15,11,14,normal 28 cases,the application of ELISA,HtrA1 in synovial fluid the expression level.Result:KOA II,Ⅲ,groupⅣthe synovial fluid HtrA1 factor expression were significantly higher than normal,there was statistically significant(P<0.01),the different cartilage degeneration patients the synovial fluid HtrA1 factor expression were also different.KOAⅡ,Ⅲ,Ⅳgroup group HtrA1 factor expression levels was not statistically significant(P>0.05).Normal control group joint fluid HtrA1 factor expression content to 67.89-208.33 pg/ml,if 208.33 pg/ml as a negative,the sensitivity of KOAⅡ,Ⅲ,Ⅳgroup were more than 85.7%.Conclusion:The HtrA1 early cartilage degeneration(KOA grade III)diagnostic sensitivity of 90%,in particular the diagnosis of patients with KOA KOA class II cartilage degeneration highest sensitivity,more than 93%. The HtrA1 description is a good indicator for the early diagnosis of cartilage degeneration(KOA grade II).
