中国医学影像学杂志
中國醫學影像學雜誌
중국의학영상학잡지
CHINESE JOURNAL OF MEDICAL IMAGING
2013年
4期
245-248
,共4页
肝硬化%超声检查%弹性成像技术%疾病模型,动物%大鼠,Wistar
肝硬化%超聲檢查%彈性成像技術%疾病模型,動物%大鼠,Wistar
간경화%초성검사%탄성성상기술%질병모형,동물%대서,Wistar
Liver cirrhosis%Ultrasonography%Elasticity imaging techniques%Disease models, animal%Rats, Wistar
目的探讨超声实时组织弹性成像(RTE)评价大鼠肝纤维化分期的应用价值.材料与方法将80只雄性Wistar大鼠采用抽签法随机分为实验组(64只)和对照组(16只),实验组经腹腔注射CCl4橄榄油溶液制作肝纤维化模型,对照组经腹腔注射生理盐水.对大鼠肝脏进行RTE检查,获取弹性图像,测量硅胶垫与肝脏的应变比.选取与弹性成像感兴趣区一致的大鼠肝组织行病理检查,并对肝纤维化程度进行分期.结果各期肝纤维化应变比分别为S0期0.4211±0.1921、S1期0.4795±0.1109、S2期0.7370±0.1397、S3期0.7980±0.1001、S4期1.0279±0.2324, S0期与S1期比较无显著差异(P>0.05),与S2、S3、S4期比较有显著差异(P<0.001);S1期与S2、S3、S4期比较有显著差异(P<0.001);S2期与S3期比较无显著差异(P>0.05),与S4期比较有显著差异(P<0.001);S3期与S4期比较有显著差异(P<0.01).应变比与病理分期呈显著正相关(r=0.799, P<0.05),随着肝纤维化程度加重,RTE应变比也随之增大.结论RTE可以半定量评价大鼠肝纤维化,并进行肝纤维化分期,有望为临床肝纤维化研究提供初步参考.
目的探討超聲實時組織彈性成像(RTE)評價大鼠肝纖維化分期的應用價值.材料與方法將80隻雄性Wistar大鼠採用抽籤法隨機分為實驗組(64隻)和對照組(16隻),實驗組經腹腔註射CCl4橄欖油溶液製作肝纖維化模型,對照組經腹腔註射生理鹽水.對大鼠肝髒進行RTE檢查,穫取彈性圖像,測量硅膠墊與肝髒的應變比.選取與彈性成像感興趣區一緻的大鼠肝組織行病理檢查,併對肝纖維化程度進行分期.結果各期肝纖維化應變比分彆為S0期0.4211±0.1921、S1期0.4795±0.1109、S2期0.7370±0.1397、S3期0.7980±0.1001、S4期1.0279±0.2324, S0期與S1期比較無顯著差異(P>0.05),與S2、S3、S4期比較有顯著差異(P<0.001);S1期與S2、S3、S4期比較有顯著差異(P<0.001);S2期與S3期比較無顯著差異(P>0.05),與S4期比較有顯著差異(P<0.001);S3期與S4期比較有顯著差異(P<0.01).應變比與病理分期呈顯著正相關(r=0.799, P<0.05),隨著肝纖維化程度加重,RTE應變比也隨之增大.結論RTE可以半定量評價大鼠肝纖維化,併進行肝纖維化分期,有望為臨床肝纖維化研究提供初步參攷.
목적탐토초성실시조직탄성성상(RTE)평개대서간섬유화분기적응용개치.재료여방법장80지웅성Wistar대서채용추첨법수궤분위실험조(64지)화대조조(16지),실험조경복강주사CCl4감람유용액제작간섬유화모형,대조조경복강주사생리염수.대대서간장진행RTE검사,획취탄성도상,측량규효점여간장적응변비.선취여탄성성상감흥취구일치적대서간조직행병리검사,병대간섬유화정도진행분기.결과각기간섬유화응변비분별위S0기0.4211±0.1921、S1기0.4795±0.1109、S2기0.7370±0.1397、S3기0.7980±0.1001、S4기1.0279±0.2324, S0기여S1기비교무현저차이(P>0.05),여S2、S3、S4기비교유현저차이(P<0.001);S1기여S2、S3、S4기비교유현저차이(P<0.001);S2기여S3기비교무현저차이(P>0.05),여S4기비교유현저차이(P<0.001);S3기여S4기비교유현저차이(P<0.01).응변비여병리분기정현저정상관(r=0.799, P<0.05),수착간섬유화정도가중,RTE응변비야수지증대.결론RTE가이반정량평개대서간섬유화,병진행간섬유화분기,유망위림상간섬유화연구제공초보삼고.
Purpose To evaluate the value of real-time tissue elastography (RTE) for assessing rat liver fibrosis grading. Materials and Methods Eighty male Wistar rats were randomly divided into experimental group (n=64) and control group (n=16). The experimental rats were intraperitoneally injected with CCl4 olive oil solution. The control group was injected with saline. RTE was implemented to measure the strain ratio between reference silicon pad and rat liver. Histopathological examination was performed to grade liver cirrhosis. Results The strain ratios were 0.4211±0.1921 for S0 stage, 0.4795±0.1109 for S1 stage, 0.7370±0.1397 for S2 stage, 0.7980±0.1001 for S3 stage, 1.0279±0.2324 for S4 stage, respectively, with significant difference between S0 and S2, S3, S4 stages (P<0.001), and between S1 and S2, S3 and S4 stages (P<0.001), and between S2 and S4 (P<0.001), S3 and S4 (P<0.001). Strain ratio and pathology classification were positively correlated (r=0.799, P<0.05). With the increased degree of liver fibrosis, RTE strain ratio was higher. Conclusion RTE cam semi-quantitatively evaluate liver fibrosis of rats, and further assess the liver fibrosis grading, which can provides initial reference for human liver fibrosis.
