中国医药导刊
中國醫藥導刊
중국의약도간
CHINESE JOURNAL OF MEDICAL GUIDE
2013年
4期
727-730
,共4页
朝鲜红参%HPLC%含量测定%指纹图谱%人参皂苷Rb1、Rb2、Rb3、Re、Rg1
朝鮮紅參%HPLC%含量測定%指紋圖譜%人參皂苷Rb1、Rb2、Rb3、Re、Rg1
조선홍삼%HPLC%함량측정%지문도보%인삼조감Rb1、Rb2、Rb3、Re、Rg1
Red ginseng from North Korea%HPLC%Determination%Fingerprint%GSinsenoside Rb1、Rb2、Rb3、Re、Rg1
目的:建立可同时测定朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re、Rg15种成分含量和指纹图谱的HPLC方法.方法:采用Discovery C18(4.6×250mm,5μm)柱,流动相为水(A)-乙腈(B)梯度洗脱;流速1.0ml?min-1,柱温为25℃,检测波长为203nm,并用计算机辅助相似性评价系统对指纹图谱进行了相似度分析.结果:朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re、Rg1的线性范围分别为1.077~5.385μg,1.046~5.230μg,0.0953~0.4765μg,1.018~5.090μg,0.973~4.865μg,相关系数分别为0.9999,0.9999,0.9995,0.9995,0.9996.人参皂苷Rb1、Rb2、Rb3、Re、Rg1平均回收率分别为96.55%、101.4%、99.08%、99.92%、96.00%;RSD分别为1.6%、1.2%、1.5%、1.2%、1.3%.不同规格的朝鲜红参之间及朝鲜红参与国产红参都具有较好的相关性.结论:该方法重复性好,可以用来同时测定朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re、Rg1五种成分的含量,14批不同规格的朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re和Rg15种成分含量存在差异,但总体而言,朝鲜红参与国产红参在人参皂苷含量和指纹图谱上无明显差异.
目的:建立可同時測定朝鮮紅參中人參皂苷Rb1、Rb2、Rb3、Re、Rg15種成分含量和指紋圖譜的HPLC方法.方法:採用Discovery C18(4.6×250mm,5μm)柱,流動相為水(A)-乙腈(B)梯度洗脫;流速1.0ml?min-1,柱溫為25℃,檢測波長為203nm,併用計算機輔助相似性評價繫統對指紋圖譜進行瞭相似度分析.結果:朝鮮紅參中人參皂苷Rb1、Rb2、Rb3、Re、Rg1的線性範圍分彆為1.077~5.385μg,1.046~5.230μg,0.0953~0.4765μg,1.018~5.090μg,0.973~4.865μg,相關繫數分彆為0.9999,0.9999,0.9995,0.9995,0.9996.人參皂苷Rb1、Rb2、Rb3、Re、Rg1平均迴收率分彆為96.55%、101.4%、99.08%、99.92%、96.00%;RSD分彆為1.6%、1.2%、1.5%、1.2%、1.3%.不同規格的朝鮮紅參之間及朝鮮紅參與國產紅參都具有較好的相關性.結論:該方法重複性好,可以用來同時測定朝鮮紅參中人參皂苷Rb1、Rb2、Rb3、Re、Rg1五種成分的含量,14批不同規格的朝鮮紅參中人參皂苷Rb1、Rb2、Rb3、Re和Rg15種成分含量存在差異,但總體而言,朝鮮紅參與國產紅參在人參皂苷含量和指紋圖譜上無明顯差異.
목적:건립가동시측정조선홍삼중인삼조감Rb1、Rb2、Rb3、Re、Rg15충성분함량화지문도보적HPLC방법.방법:채용Discovery C18(4.6×250mm,5μm)주,류동상위수(A)-을정(B)제도세탈;류속1.0ml?min-1,주온위25℃,검측파장위203nm,병용계산궤보조상사성평개계통대지문도보진행료상사도분석.결과:조선홍삼중인삼조감Rb1、Rb2、Rb3、Re、Rg1적선성범위분별위1.077~5.385μg,1.046~5.230μg,0.0953~0.4765μg,1.018~5.090μg,0.973~4.865μg,상관계수분별위0.9999,0.9999,0.9995,0.9995,0.9996.인삼조감Rb1、Rb2、Rb3、Re、Rg1평균회수솔분별위96.55%、101.4%、99.08%、99.92%、96.00%;RSD분별위1.6%、1.2%、1.5%、1.2%、1.3%.불동규격적조선홍삼지간급조선홍삼여국산홍삼도구유교호적상관성.결론:해방법중복성호,가이용래동시측정조선홍삼중인삼조감Rb1、Rb2、Rb3、Re、Rg1오충성분적함량,14비불동규격적조선홍삼중인삼조감Rb1、Rb2、Rb3、Re화Rg15충성분함량존재차이,단총체이언,조선홍삼여국산홍삼재인삼조감함량화지문도보상무명현차이.
@@@@Objective:To establish a method of fingerprint and simultaneous determination of ginsenoside Rb1、Rb2、Rb3、Re and Rg1 in red ginseng from North Korea by HPLC.Methods:The column was Discovery C18 (4.6×250mm,5μm),the mobile phase consisted of water(A) and acetonitrile (B) with linear gradient elution,the flow rate was 1.0ml?min-1,the column temperature was 25℃,the detection wavelength was 203nm;the fingerprint was analysised by similar computer-assisted.Results:The linear range of ginsenoside Rb1、Rb2、Rb3、Re and Rg1 were 1.077~5.385μg (r=0.9999),1.046~5.230μg(r=0.9999),0.0953~0.4765μg(r=0.9995),1.018~5.090μg(r=0.9995),0.973~4.865μg(r=0.9996),Their average recoveries were 96.55%、101.4%、99.08%、99.92%、96.00%with RSD=1.6%、1.2%、1.5%、1.2%and 1.3%,respectively(n=5).The similarity of different sector red ginsengs from North Korea and china were good. Conclusion:The method is good reproducible and can be used to determine ginsenoside Rb1、Rb2、Rb3、Re and Rg1 in red ginseng from North Korea,the saponin contents in fourteen red ginseng from North Korea were different.There was no difference in fingerprint between red ginseng from North Korea and china.
