CAJ | 학술논문

目的:研究胃癌及癌旁组织中E-钙粘附素(E-cadherin)和环氧合酶(cyclooxygenase:COX)-2的表达,探讨蛋白表达与临床病理指标及与癌旁黏膜表达的关系.方法:采用组织芯片及免疫组织化学技术,对胃癌组织121例及118例和癌旁组织113例及111例.分别检测E-cadherin及COX-2,并分析二者蛋白表达情况与临床病理指标相关性.结果:胃癌E-cadherin阳性率为76.9%(93/121),其表达降低与肿瘤大体分型(P=0.014)、Lauren分型(P<0.001)、肿瘤分化(P=0.004)及pTNM分期(P=0.024)等显著相关,分化差分期晚的肿瘤E-cadherin表达要显著低于分化好分期早的肿瘤.癌旁黏膜组织E-cadherin均呈阳性表达,与肿瘤无显著相关.COX-2蛋白阳性率为38.1%(45/118),其过表达与淋巴结转移相关(P=0.048),有淋巴结转移的表达率高,与其它病理指标无明显相关性.结论:E-cadherin及COX-2分别从肿瘤分化、分期及淋巴结转移等方面预测胃癌患者预后,二者互补,联合应用可做为临床预后判断的参考指标.
목적:연구위암급암방조직중E-개점부소(E-cadherin)화배양합매(cyclooxygenase:COX)-2적표체,탐토단백표체여림상병리지표급여암방점막표체적관계.방법:채용조직심편급면역조직화학기술,대위암조직121례급118례화암방조직113례급111례.분별검측E-cadherin급COX-2,병분석이자단백표체정황여림상병리지표상관성.결과:위암E-cadherin양성솔위76.9%(93/121),기표체강저여종류대체분형(P=0.014)、Lauren분형(P<0.001)、종류분화(P=0.004)급pTNM분기(P=0.024)등현저상관,분화차분기만적종류E-cadherin표체요현저저우분화호분기조적종류.암방점막조직E-cadherin균정양성표체,여종류무현저상관.COX-2단백양성솔위38.1%(45/118),기과표체여림파결전이상관(P=0.048),유림파결전이적표체솔고,여기타병리지표무명현상관성.결론:E-cadherin급COX-2분별종종류분화、분기급림파결전이등방면예측위암환자예후,이자호보,연합응용가주위림상예후판단적삼고지표.
Objective:This work aimed to evaluate the expressions of E-cadherin and cyclooxygen?ase-2 (COX-2) in primary gastric carcinoma and adjacent mucosa, and to investigate the correlation between abnormal expression of the said proteins and the clin-ico-pathological features of gastric adenocarcinoma. Methods:Immunohistochemical staining was used to determine the expression of E-cadherin and COX-2 on tissue microarray slices obtained from gastric carcinoma and adjacent mucosa of over 100 cases from the Cancer Institute and Hospital of Chinese Academy of Medical Science between February 2005 and October 2006. Results:The positive expressions of E-cadherin and COX-2 were 76.9%(93/121) and 38.1%(45/118), respectively, in gastric carcinoma, whereas E-cadherin expression was 100%in 113 cases with adjacent mucosa. The positive expression of E-cadherin was significantly correlated with clini-cal characteristics such as gross type (P=0.014), differentiation (P=0.004), and pTNM stage (P=0.024). COX-2 expression was higher in gastric cancer cases with lymph node me?tastasis than those without, and the result was significantly different (P=0.048). Conclu-sion:E-cadherin is a tumor-inhibiting factor and COX-2 is a carcinogen. Positive expression of E-cadherin and negative expression of COX-2 are correlated with better prognosis. E-cadherin and COX-2 may thus be used as potential markers of tumor progression and prognosis.