CAJ | 학술논문

目的:乳腺浸润性微乳头状癌(IMPC)具有特殊的组织学形态特征及淋巴结转移率高的生物学特性.本研究旨在通过比较蛋白质组学技术筛选,并验证乳腺IMPC与最常见的非特殊型浸润性导管癌(IDC-NOS)间差异表达的蛋白,从蛋白质角度研究探讨乳腺IMPC的特殊组织学形态及生物学行为机制.方法:双向电泳筛选1例乳腺IMPC和1例乳腺IDC-NOS冻存组织的蛋白表达差异点,质谱鉴定其所对应的蛋白质,免疫组织化学染色检测验证其在43例IMPC和30例IDC-NOS组织切片中的表达.结果:筛选出表达稳定差异的蛋白-热休克蛋白27(Hsp27),且Hsp27在IMPC中表达上调.免疫组织化学染色验证了Hsp27主要表达于肿瘤细胞质中,其在IMPC中的表达明显高于IDC-NOS(Z=-3.236,P=0.001),并与ER(r=0.319,P=0.037)及淋巴结转移数(r=0.444,P=0.003)呈正相关,而与患者年龄、病理学分期、PR和HER-2表达无明显相关性.结论:Hsp27在乳腺IMPC中过表达,且与淋巴结转移数呈正相关,提示Hsp27可能在IMPC淋巴结转移以及特殊的病理形态形成中起着重要作用.
목적:유선침윤성미유두상암(IMPC)구유특수적조직학형태특정급림파결전이솔고적생물학특성.본연구지재통과비교단백질조학기술사선,병험증유선IMPC여최상견적비특수형침윤성도관암(IDC-NOS)간차이표체적단백,종단백질각도연구탐토유선IMPC적특수조직학형태급생물학행위궤제.방법:쌍향전영사선1례유선IMPC화1례유선IDC-NOS동존조직적단백표체차이점,질보감정기소대응적단백질,면역조직화학염색검측험증기재43례IMPC화30례IDC-NOS조직절편중적표체.결과:사선출표체은정차이적단백-열휴극단백27(Hsp27),차Hsp27재IMPC중표체상조.면역조직화학염색험증료Hsp27주요표체우종류세포질중,기재IMPC중적표체명현고우IDC-NOS(Z=-3.236,P=0.001),병여ER(r=0.319,P=0.037)급림파결전이수(r=0.444,P=0.003)정정상관,이여환자년령、병이학분기、PR화HER-2표체무명현상관성.결론:Hsp27재유선IMPC중과표체,차여림파결전이수정정상관,제시Hsp27가능재IMPC림파결전이이급특수적병리형태형성중기착중요작용.
Objective: Compared with invasive ductal carcinoma-not otherwise specified (IDC-NOS), invasive micropapillary carcinoma (IMPC) shows more distinctive morphologic characteristics and a biological feature with high incidence of axillary lymph node metastases. This study used comparative proteomics technology to find the differentially expressed proteins between IMPC and IDC-NOS, followed by verification. Afterward, the mechanisms of the special pathological morphology and biological behavior of IMPC was explored from the point view of proteomics. Methods:Candidate molecules were obtained by examining the differential expression spots in the frozen tissues of one IMPC and one IDC-NOS. Two-dimensional polyacrylamide gel electrophoresis and mass spectrum were used as tools for analysis. The immunohistochemical stain assay was used to verify the expression of these candidate molecules in the tissue sections of 43 IMPC and 30 IDC-NOS frozen samples. Results:One differentially expressed protein, namely, Heat shock protein 27 (Hsp27), was screened out. The expression of Hsp27 was up-regulated in IMPC compared with that in IDC-NOS. Hsp27 was mainly expressed in the plasma of tumor cells, and the expression was significantly higher in IMPC than in IDC-NOS (Z=-3.236, P=0.001). The expression of Hsp27 positively correlated with the estrogen receptor (r=0.319, P=0.037) and metastasis in lymph nodes (r=0.444, P=0.003). No correlation was found between Hsp27 and the patient's age, pathologic stage, and progesterone receptor or human epidermal growth factor receptor 2. Conclusion:Hsp27 shows over-expression in IMPC and is positively correlated with metastasis in lymph nodes. Hsp27 may play an important role in the formation of the special pathological morphology of IMPC, as well as the high rate of lymph node metastasis.